removed from the solution by adding about 3 volumes of alcohol. The 

 precipitate settled, leaving a clear supernatant liquid ; adding mort_ 

 alcohol produced no further precipitate. It was then filtered and 

 washed in alcohol and the filtrate reserved. 



After drying, the above precipitate was obtained as a heavy, white, 

 amorphous powder. It was free from inorganic phosphorus, but con- 

 tained barium and after combustion the ash gave a heavy yellow pre- 

 cipitate with ammonium molybdate. This substance was purified, as 

 will be described later. 



The filtrate from the above precipitate was again evaporated on 

 the water-bath nearly to dryness, taken up in hot water, filtered and 

 mixed with alcohol and ether, On scratching with a glass rod a sub- 

 stance began to crystallize in needles. It was allowed to stand in 

 the ice chest over night. The crystals were then filtered, washed in 

 alcohol and ether and dried in the air. Yield, 0.25 gram. The sub- 

 stance was recrystallized four times in the same manner and was 

 finally obtained in colorless needles free from -water of crystallization. 

 It gave the reaction of Scherer and melted at 222° (uncorrected). It 

 was, therefore, no doubt pure inosite. This was further confirmed by 

 the analysis : 



0.1215 gram substance gave 0.0737 gram H 2 and 0.1780 gm. CO„. 



Found : C= 39.95 ; H=6.78 per cent. 



For C 6 H 12 O = 180; 



Calculated: C= 40.00; H = 6.66 per cent. 



PURIFICATION OF THE BARIUM AND PHOSPHORUS CON- 

 TAINING PRECIPITATE REMOVED FROM THE 

 INOSITE SOLUTION WITH ALCOHOL 



The substance mentioned above, precipitated with alcohol, was 

 apparently the barium salt of an organic phosphoric acid. It was 

 very soluble in water and was not precipitated from the aqueous solu- 

 tion by barium hydroxide. The dry substance weighed 1.2 grams. It 

 was dissolved in a small quantity of water, a few drops of dilute hydro- 

 chloric acid added and 10 cc. of N/1 barium chloride. The solution 

 was heated to boiling and alcohol added until a slight cloudiness was 

 produced. On standing in the cold over night a small amount of a 

 hard crust had separated on the bottom of the flask. This was removed 

 and the solution again heated and more alcohol added when a further 

 quantity separated in the same way. The substance was finally fil- 

 tered and washed thoroughly in 80 per cent, alcohol, alcohol and ether, 

 and dried in the air. Without further purification the substance was 

 analyzed after drying at 105° in vacuum over phosphorus pentoxide. 



58 



