40 STUDIES IN SPERMATOGENESIS. 



somes is seen in figure 102. Equatorial plates of the second division, 

 one containing the small chromosome {b), are shown in figure 103. A 

 prophase of the same division (fig. 104) proves that the small chromo- 

 some divides quantitatively like the others. It was interesting to find 

 here and there in this material whole cysts in which the nuclei were 

 like those described by Paulmier ('99) for Anasa trisiis (plate xiii, fig. 

 14) as cells which were being transformed to serve as food for the 

 glowing spermatids (figs. 105, 106). The only occasional appearance 

 of these cysts seems to me to preclude their being a special dispen- 

 sation to furnish the spermatids with nutrition daring their transfor- 

 mation. Their appearance and size make me suspect that they are 

 giant spermatids due to the failure of one of the spermatogonial or 

 spermatocyte mitoses. The smaller chromatin body seems to corre- 

 spond to that described for the spermatids of Odontota dorsalis. 



Euphoria inda (Family Scarabaeidae). 



Of Euphoria inda only one male was captured, but the numerous 

 testes furnished abundant material in desirable stages. The sperma- 

 togonial equatorial plate (fig. 107) contains 20 chromosomes of which 

 the two smallest (/ and.?) form the unequal pair. The resting sperma- 

 togonium contains a two-lobed plasmosome (fig. 108). The growth 

 stages are similar to those in Tenebrio in showing no distinct bouquet 

 stage, but there is a spireme stage in which the heterochromosome pair 

 is clearly seen (fig. log). Figure no (plate xi) is an early prophase, 

 and figure in one in which the unequal pair appears with a tetrad and 

 several dumb-bell forms. The prophase of the spindle, as in Odontota, 

 is much elongated (fig. 112). In figures 113-116 the small heterochro- 

 mosome pair is shown in various positions with reference to the other 

 chromosomes of the metaphase of the first spermatocyte.. Figure 117 

 shows it more deeply stained than the others in the equatorial plate. 

 This pair divides in advance of the others, and the larger and smaller 

 elements are plainly seen nearer the poles in anaphase than the other 

 univalent chromosomes (figs. 1 18-120). Daughter plates of the first 

 spermatocyte are shown in figure 121, and equatorial plates of the 

 second spermatocyte in figure 123. Figure 122 shows the telophase 

 of the first division with the spindle for the second division forming. 

 In figures 124 and 125 we have daughter plates of the two classes of 

 second spermatocytes, showing the content of the two equal classes of 

 dimorphic spermatozoa, as this was shown in Tenebrio. Figures 126 

 and 127 are anaphases showing the division of the heterochromosomes 

 (/ and s). Figures 128-130 are early stages in the development of the 

 spermatid showing the chromatin nucleolus («) in various phases. 



