324 DOMESTICATED TROUT. 



Impregnating Eggs.* 



1. Take ripe eggs with little milt and note the percent- 

 age of impregnation. 



2. Take ripe eggs with abundant milt and note as before. 



3. Try immature eggs with good and sufficient milt. 



4. Try ripe eggs with poor milt. 



5. After mixing milt and eggs, add water at 36°, at 45 , 

 at 60°, and compare results. 



6. Use milt that has been taken in a dry phial and corked 

 up 24 hours, 48 hours, 96 hours, and compare results. 



7. Use milt that has been bottled up and sent by mail 

 100 miles, 500 miles, 1,000 miles. 



8. Use milt that has been forced f by putting male in 

 warm water, and note the degree of impregnation. 



9. Use milt that has been frozen. 



10-12. Repeat experiments 6, 7, 9, using eggs instead 

 of milt. 



13. Put ripe eggs with milt that has been exposed to 

 the air 5 minutes, 20 minutes, 30 minutes. 



14. Put good milt with eggs that have been exposed to 

 the air 5 minutes, 20 minutes, 30 minutes. 



15. Use ripe eggs with milt that has been diluted with 

 water 2 minutes, 5 minutes, 20 minutes. 



16. Use good milt with eggs that have been kept in 

 water 2 minutes, 5 minutes, 20 minutes, 30 minutes. 



17. Compare the percentage of impregnation of eggs 

 taken in Ainsworth's races and Collins's roller box with 

 those taken by manual pressure. 



Experiments in general with Eggs. 



18. Pack eggs in wet moss (Sphagnum) as soon as taken, 

 and examine when nearly ready to hatch, and note the 

 mortality. 



* See p. 104. f See p. 115, note. 



