198 ACTION OF COAL-TAR PRODUCTS, 
lus by the same amount in thirty minutes. Boer found malachite 
green to be still more effective than methyl violet. In his experi- 
ments upon bouillon cultures twenty-four hours old, with two hours’ 
exposure to the action of the disinfectant, he obtained the following 
results : 
MALACHITE GREEN. 
Restrains Destroys 
development. vitality. 
Anthrax bacillus .. 2... .. 2... cece eee ee 1: 120000 1: 40000 
Diphtheria bacillus ........ cece cece eee eee: 1: 40000 1:8000 
Glanders bacillus .......... 0... ce eee e eee e ee ene 1: 5000 1:300 
Typhoid bacillus 2. 6... . eee cee eee eee ee 1: 5000 1 :300 
Cholera spirillum........ 6.0. cc eee eee ee oe 1: 100000 1: 5000 
METHYL VIOLET (PYOKTANIN). 
Restrains Destroys 
development. vitality. 
Anthrax bacillus.......... cece eee ee cece eee 1 :'70009 1:5000 
Diphtheria bacillus....... .ceeeee cree ener e ees 1:10000 1: 2000 
Glanders bacillus... ... cece cece ee crete cer eeeee 1: 2500 1-:150 
Typhoid bacillus...... .....4. Sra tail tila Su mora races 1: 2500 1:150 
Cholera spirillum........ eee ee eee ee ee cee : 1: 30000 1:1000 
Aniline Oil.—According to Riedlin, the addition of 1:5 of ani- 
line water prevents the development of all bacteria in nutrient gelatin. 
Aromatic Products of Decomposition.—Klein has tested the 
germicidal power of phenylpropionic and phenylacetic acids. He 
finds that anthrax spores resist both of these acids, in the proportion 
of 1:400, for two days, but in the absence of spores anthrax bacilli 
are quickly killed by a solution of this strength. Certain non-patho- 
genic micrococci were not killed by exposure for twenty-five minutes 
to 1:200. The caseous matter of pulmonary tuberculosis infected 
guinea-pigs after exposure for ninety-six hours to 1 : 200, 
Aseptol,—A ten-per-cent aqueous solution kills anthrax spores in 
ten minutes, and a three- to five-per-cent solution is a reliable disin- 
fectant in the absence of spores (Hueppe). 
Benzene, C,H,.—Exposure in benzol for twenty days failed to 
destroy the vitality of anthrax spores (Koch). 
Camphor.—Alcohol saturated with camphor has no effect upon 
the virus of symptomatic anthrax (Arloing, Cornevin, and Thomas) 
The experiments of Cadéac and Meunier show that camphor (oil 
of, or tincture?) has but little germicidal power. The typhoid ba- 
