PYOGENIC BACTERIA. : 377 
a pure culture made directly into the circulation, and there is evi- 
dence that the pathogenic potency of this micrococcus may vary 
considerably as a result of conditions relating to its origin and culti- 
vation in the animal body or in artificial media. When injected in 
considerable quantities it may be obtained in cultures from the 
urine, but not sooner than six or eight hours after the injection, and 
not until the formation of purulent foci in the kidneys has already 
occurred (Wyssokowitsch). 
The pyogenic properties of this micrococcus have been demon- 
strated upon man by the experiments of Garré, of Bockhart, and of 
Bumm. The first-named observer inoculated a small wound at the 
edge of one of his finger nails with a minute quantity of a pure cul- 
ture, and a subepidermal, purulent inflammation extending around 
Fia. 81.—Vertical section through a subcutaneous abscess caused by inoculation witb staphylo- 
cocci, in the rabbit, forty-eight hours after infection; margin towards the normal tissue. x 950. 
(Baumgarten.) 
the margin of the nail resulted from the inoculation. Staphylococ- 
cus aureus was recovered in cultures from the pus thus formed. A 
more extensive and extremely satisfactory experiment was subse- 
quently made by Garré, who applied a considerable quantity of a 
pure culture obtained from the above-mentioned source—third gene- 
ration—to the uninjured skin of his left forearm. At the end of 
four days a large carbuncle, surrounded by isolated furuncles, de- 
veloped at the point where the culture had been applied. This ran 
the usual course, and it was several weeks before it had completely 
healed. No less than seventeen scars remained to give evidence of 
the success of the experiment. 
In Bockhart’s experiments a similar but milder result was ob- 
tained, the conditions having been somewhat different. A small 
