394 PYOGENIC BACTERIA. 
Turro also claims to have produced specific urethritis in dogs by 
inoculation with his cultures. Heiman (1895) as a result of an ex- 
tended experimental research, arrives at the conclusion that “the 
diplococcus described by Turro in connection with his acid media is 
not the gonococcus.” His inoculation experiments in dogs, made 
with pure cultures of the gonococcus, gave an entirely negative result. 
For the cultivation of the gonococcus, Heiman recommends a medium 
made from “chest serum” obtained from a patient suffering with 
hydrothorax or acute pleurisy. This was found to be superior to 
placenta serum, sheep-blood serum, or peritoneum serum, because of 
the great amount of serum albumin which it contains. Two per 
cent of agar, one per cent of peptone, and one-half per cent of sodium 
chloride were added to the chest serum, and the medium was sterilized 
by “fractional sterilization.” 
Fie. 87.—Gonorrhoeal conjunctivitis, second day of sickness; section through the mucous mem- 
brane of upper eyelid; invasion of the epithelial layer by gonococci. (Bumm.) 
Schrétter and Winkler (1890) report their success in cultivating 
the gonococcus upon albumin from the egg of the pewit—“‘ Kibitz.” 
In the culture oven at 38° C. a thin, transparent, whitish layer was 
already visible at the end of six hours and rapidly extended ; the 
growth was less abundant at the end of three days, and had entirely 
ceased by the fifth day. Attempts to cultivate the same microér- 
ganism in albumin from hens’ eggs gave a negative result. 
Aufuso (1891) has cultivated the gonococcus in fluid obtained 
from the knee joint in a case of chronic synovitis, but failed to culti- 
vate it in the fluid of ascites. A culture of the twelfth generation 
made upon the culture medium mentioned, solidified by heat, was 
introduced into the urethra of a healthy man and gave rise to a 
characteristic attack of gonorrhea. 
Development does not occur below 25° or above 38° GC. The 
writer has shown that a temperature of 60° C. maintained for ten 
minutes destroys the infective virulence of gonorrhceal pus. 
Pathogenesis.—That the gonococcus is the cause of the specific 
inflammation and purulent discharge characteristic of gonorrhea is 
now generally admitted upon the experimental evidence obtained by 
