BACTERIA IN CROUPOUS PNEUMONIA. 405 
one part of ninety-five-per-cent alcohol to three parts of virus), and that 
both have a restraining influence upon the development of this microdrgan- 
ism in culture fluids.” 
The following results were obtained by the writer in his experi- 
ments (1881 and 1883) to determine the germicidal and antiseptic 
value of the agents named, as tested upon this micrococcus. 
Alcohol.—A twenty-four-per-cent solution was effective upon 
bouillon cultures in two hours. 
Boric Acid.—A saturated solution failed to destroy vitality after 
two hours’ exposure, but | : 400 restrained development. 
Carbolic Acid.—A one-per-cent solution destroys vitality in two 
hours, and 1 : 500 restrains development. 
Cupric Sulphate destroys the virulence of the coccus in the 
blood of a rabbit in the proportion of 1 : 400 in half an hour. 
Ferric Sulphate failed to destroy vitality in a saturated solution, 
but restrained development in the proportion of 1 : 200. 
Hydrochloric Acid destroys the virulence of the blood of a rab- 
bit containing this micrococcus in the proportion of 1 : 200. 
Iodine, in aqueous solution with potassium iodide, destroys vital- 
ity in the proportion of 1: 1,000 and prevents development in 1: 4,000. 
Mercuric Chloride.—One part in forty thousand prevents the 
development of this micrococcus, and 1 : 20,000 was found to destroy 
vitality in two hours. 
Nitric Acid.—One part in four hundred destroyed the virulence 
of rabbit’s blood containing this micrococcus. 
Caustic Potash.—A two-per-cent solution destroyed vitality in 
two hours. 
Potassium Permanganate.—A two-per-cent solution,destroyed 
the virulence of rabbit’s blood containing this coccus. 
Salicylic Acid, dissolved by the addition of sodium biborate.— 
A solution of 1 : 400 prevented development. 
Sulphuric Acid.—One part in two hundred destroys vitality, and 
1: 800 prevents development. 
In a paper by Bordoni-Uffreduzzi relating to the resisting power 
of pneumonic virus for desiccation and light, the following results are 
given: Pneumonic sputum attached to cloths, when dried in the air 
and exposed to diffuse daylight, retained its virulence, as shown by 
injection in rabbits, for a period of nineteen days in one series of ex- 
periments and for fifty-five days in another. Exposed to direct sun- 
light the same material retained its virulence after twelve hours’ 
exposure. Cultures have far less resistance, and the protection 
afforded by the dried albuminous material in which the micrococci 
were embedded, in the experiments referred to, probably accounts 
for the virulence being retained so long a time. 
