NOT DESCRIBED IN SECTIONS V. AND VI. 413 
bubble forms near the surface of the gelatin ; very slight liquefac- 
tion occurs in the immediate vicinity of the line of growth, and after 
atime the grayish-white thread sinks into an irregular mass, lying 
at the bottom of the puncture. Upon nutrient agar scarcely any de- 
velopment occurs. Upon potato the growth is abundant, in the form 
of a pale-yellow, circular layer, and the culture gives off the peculiar 
odor above described. 
Pathogenesis.—When inoculated into guinea-pigs general infec- 
tion and death result. In sheep and goats it produces a local in- 
flammatory cedema and sometimes necrosis of the tissues. In horses 
inoculated subcutaneously an inflammatory cedema first occurs, fol- 
lowed at the end of from four to six weeks by the development of new 
growths in the connective tissue, resembling the tumors found in 
cases of the disease in the animal from which the micrococcus in 
question was first cultivated. These tumors contain characteristic 
mulberry-like conglomerations of colonies made up of the coccus. 
MICROCOCCUS OF MANFREDI. 
Synonym.—Micrococcus of progressive granuloma formation. 
Obtained by Manfredi (1886) from the sputum of two cases of 
croupous pneumonia following measles. 
Morphology.—Oval micrococci, having a diameter of 0.6 to 1.0 
and from 1.0 to 1.5 in length ; usually associated in pairs, and oc- 
casionally in short chains containing three or four elements. 
Stains with the aniline colors and by Gram’s method. 
Biological Characters.—Aérobic; does not liquefy gelatin. 
Upon gelatin plates forms small, spherical colonies, at first grayish- 
white, which spread out upon the surface as thin, transparent plates, 
which by transmitted light have a bluish, by reflected light a pearl- 
gray color. Later these become. thicker and have a pearly lustre. 
Under the microscope (forty to fifty diameters) the colonies are seen 
to be slightly granular and the margins have an irregular outline. 
In gelatin stab cultures a scanty growth occurs along the line of 
puncture, and a rather thin and limited growth about the point of 
inoculation. Upon blood serum a thin, greenish-yellow layer, which 
has irregular margins and a slightly granular, shining surface, is 
developed. The growth upon potato, at 37° C., is scanty, and con- 
sists of a very thin, moist layer, which has a yellowish color and is 
slightly granular. Growth occurs in favorable media—bouillon, 
gelatin—at temperatures of 18° to 48° C., but ceases at a temperature 
of 48° to 50° C. 
Pathogenesis.—Pathogenic for dogs, rabbits, guinea-pigs, mice, 
and birds. In mammals the principal pathological appearance re- 
sulting from infection consists in the formation of ‘‘ granulation tu- 
