492 BACILLI IN CHRONIC INFECTIOUS DISEASES. 
can easily be obtained in pure cultures from the interior of suppurat- 
ing nodules and glands which have not yet opened to the surface, 
and the same material will give successful results when inoculated 
into susceptible animals. But the discharge from the nostrils or from 
an open ulcer contains comparatively few bacilli; and as these are 
associated with various other bacteria which grow more readily in 
our culture media, it is not easy to obtain pure cultures, by the plate 
method, from such material. 
In the guinea-pig subcutaneous inoculation is followed in four or 
five days by tumefaction at the point of inoculation, and after a time 
a prominent tumor with caseous contents is developed ; ulceration of 
the skin follows, anda chronic, purulent ulcer with irregular, indu- 
rated margins results; after a time this may cicatrize. Meanwhile 
the lymphatic glands become involved, and the symptoms of general 
Fig. 123.—Section through a glanders nodule in liver of field mouse. Tissue 250. Bacilli 
x 500. (Baumgarten.) 
infection are developed at the end of four or five weeks ; the glands 
suppurate, and in males the testicles are also involved ; finally a dif- 
fuse inflammation of the joints occurs, and death results from ex- 
haustion. In the guinea-pig the specific ulcers upon the nasal mu- 
