IN SUSCEPTIBLE ANIMALS. 517 
by transmitted light and are bluish-white by reflected light; the superficial 
colonies are translucent, with a bluish-white lustre. 2 
On potato, at 22° C., at the end of forty eight hours there is a thin, dirty- 
yellow growth of limited extent; at the end of ten days there is a thin, 
gamboge yellow layer and little masses of the same color; the growth is 
quite thin, with irregular outlines, and is confined to the vicinity of the 
impfstrich. 
Grows in nutrient agar containing 0.2 per cent of hydrochloric acid. 
Thermal death point 55° C. Grows in agua coco without forming gas, and 
causes this liquid and bouillon to become slightly translucent—not milky. 
_ Pathogenesis.—Pathogenic for guinea-pigs, less so for rabbits. Guinea- 
pigs inoculated subcutaneously with a few drops of a pure culture die in ten 
or twelve bours from general infection. There is usually a considerable 
effusion of bloody serum in the vicinity of the point of inoculation, and the 
spleen is more or less enlarged. 
BACILLUS CRASSUS SPUTIGENUS. 
Obtained by Kreibohm (1886) from the sputum of two individuals, and 
once in scrapings from the tongue. 
Morphology.—Short, thick bacilli, of oblong form, with rounded corners, 
often bent or twisted—‘‘sausage-shaped.” Immediately after division the 
bacilli are about one-half longer than they are broad, but before dividing 
Fie. 139.—Bacillus crassus sputigenus, from blood of mouse. x 700. (Fligge.) 
again they may <ttain a length of three to four times the breadth. Irregular 
forms with swollen ends or uneven contour are frequently seen. 
This bacillus is quickly stained by the ordinary aniline colors and also 
by Gram’s method. 
Biotogical Characters.—An aérobic, non-liquefying (non-motile % ba- 
cillus. Grows in various culture media at the room temperature—more 
navily in the incubating oven. ‘‘Appears to form spores at 35° C.” 
(Fligge). 
Taaeris plates, at the end of thirty-six hours, grayish-white colonies are 
developed, which soon reach the surface of the gelatin and spread out as 
round, viscid, grayish white drops, which project considerably above the 
surface of the culture medium. Undera low magnifying power recent colo- 
