IN SUSCEPTIBLE ANIMALS. 523 
tures, at 18° to 20° C., liquefaction has already commenced along the line of 
puncture at the end of twelve hours, and at the end of thirty-six to forty- 
eight hours half of the gelatin is liquefied in funnel shape; on the third or 
fourth day the gelatin is completely lique- 
fied, and a thick, white, flocculent deposit 
is seen at bottom of the tube. In glycerin- 
agar, at 87° C., a slight, bluish, diffuse 
fluorescence is seen upon the surface at the 
end of twelve hours, and soon after a luxu- 
riant growth, which soon covers the entire 
surface, is developed; at the end of twenty- 
four to thirty-six hours large gas bubbles 
begin to form in the agar; gradually the 
fluorescence disappears, the surface growth 
becomes thicker and hasa dirty-gray color 
which changes later to brownish. “Blood 
serum is a favorable medium and is rapidly 
liquefied by this bacillus. Upon potato the 
growth is most characteristic. At the end 
of twelve hours a thin, straw-yellow layer 
is developed along the impfstrich; this 
gradually becomes yellow, and at the end 
Fie. 142.—Bacillus hydrophilus fus- 
cus, in blood of triton. (Sanarelli.) 
of four to five days hasa brown color, resembling that of the glanders bacil- 
lus upon potato. 
Pathogenesis.—Pathogenic for frogs, toads, lizards, and oth ‘“‘cold- 
blooded” animals; also for guinea-pigs, rabbits, dogs, 
cats, mice, chickens, and pigeons. When a few drops of 
Fig. 143.—Bacillus 
hydrophilus fuscus; 
culture in nutrieut 
gelatin, end of six- 
teenhours. (Sana- 
relli.) 
a bouillon culture are injected into the muscles of the 
thigh, swelling and redness at the point of inoculation 
are quickly developed, and death usually occurs in eight 
to ten hours. The bacilli are found in great numbers in 
the blood and in all of the organs. Guinea pigs die from 
general infection within twelve hours after receiving a 
subcutaneous injection of a small amount of a pure cul- 
ture; the spleen is enlarged and the liver and spleen hy- 
pereemic; an extensive inflammatory cedema in the vicin- 
ity of the inoculation wound is frequently observed; the 
bacilli are very numerous in the blood and in all the or- 
gans. Rabbits die in five to six hours from an intravenous 
injection. Adult dogs are immune, but new-born dogs 
(three to four days old) die infallibly, after receiving a 
subcutaneous injection of a small quantity of a pure cul- 
ture, in twelve to thirty-six hours. Young cats also suc- 
cumb to similar inoculations. Chickens and pigeons die 
within five to seven hours after receiving an intravenous 
injection, but resist subcutaneous injections. 
BACILLUS TENUIS SPUTIGENUS. 
Obtained by Pansini (1890) from sputum. 
Morphology. Short bacilli, usually in pairs and sur- 
rounded by a capsule. 
Stains by Gram’s method. 
Biological Characters.—An aérobic, non-liquefying, 
non-motie bacillus. Grows in nutrient gelatin at the 
room temperature. Develops abundantly on potato. 
Coagulates milk and produces an acid reaction in this 
medium. 
Pathogenesis.— Pathogenic for rabbits and white rats; not for guinea- 
pigs or for white mice (in small doses). 
