NOT DESCRIBED IN PREVIOUS SECTIONS, 547 
very markedly in this medium from any other bacilli examined. The same 
changes are to be seen at room temperature, but more slowly. 
Egg-Albumin: Plain.—Twenty-four hours at 37° C., ‘yellowish-white, 
very profuse growth all along the needle track ; yellowish-green spreading 
out from it almost to sides of tube, and in the condensation water as well. 
The growth has no especial distinguishing characteristics. Irregular lique- 
faction occurs, but the growth at no time differsin any marked way from 
other varieties of the Bacillus pyocyaneus. 
_ Blood Serum.—Twenty-four hours at 37° C. shows flat, moist colony 
with bluish-green fluorescence in its neighborhood. Liquefaction begins 
early and goes on slowly until complete in from one to two weeks, with an 
increasing intensity of color which becomes markedly blue, and eventually 
almost black. 
Milk.—Behaves as do the other bacteria. 
BEHAVIOR TO TEMPERATURE.—Grows at 15°-25° C. slowly; much more 
freely at 35°-38° C., when it produces the color more quickly. 
RaPIDITY OF GROWTH.—Moderate. 
SPORE-PRODUCTION.—Not observed. 
NEED oF AIR.—Does not grow undermica. Facultatively anaérobic, but 
does not produce color except with free access of oxygen. 
Gas-PRODUCTION.—Produces faint foul odor. 
BEHAVIOR TO GELATIN.—Liquefies gelatin slowly. 
CoLor-PRropuction.—Produces a bluish-green color which in old cul- 
tures changes almost to a black. Upon the addition of acids (both vegetable 
and mineral) to cultures the color changes to red, and upon the addition of 
alkalies abright grass-green appears. This reaction is best seen in bouillon 
and gelatin cultures, but occurs in other media as well, notably blood-serum. 
BEHAVIOR To ANILINE DyEs.—Stains easily and well with any of the 
aniline dyes usually employed, and by Gram’s method. 
Microscopic APPEARANCE IN DIFFERENT Mep1a.—Under the micro- 
scope, its general appearance on various media is of a rod larger than the 
Bacillus pyocyaneus. In peptone cultures this difference is verv marked. 
In this case, the Bacillus pyocyaneus tested appeared as very short, oval, 
bacilli, almost like micrococci, while the new bacillus showed as a long, 
fine rod, from four to six times as long as broad—length about one-half the 
diameter of a red-blood corpuscle—and arranged sometimes two or three 
end to end. These same cultures transferred to gelatin became indistin- 
guishable from each other in size. 
PaTHOGENESIS.—Injections of small quantities (0.5 centimetre) of a bouil- 
lon culture twenty-four hours old into the abdominal cavity of rabbits and 
guinea-pigs, killed fifty per cent in from twenty-four to thirty-six hours. 
Autopsy showed general congestion of abdominal viscera, slight effusion into. 
the peritoneal cavity, and cover-glass preparations and cultures showed the 
bacilli in the effusion in the abdominal cavity, as well as in the blood from. 
the heart and various organs. 
BACILLUS OF FIOCCA. 
Found by Fiocca in the saliva of cats and dogs. 
Closely resembles the influenza bacillus of Pfeiffer and of Canon, 
Morphology.—Resembles the bacillus of rabbit septicaemia, but is only 
half as large—from 0.2 to 0.33 # in breadth. The length is but little greater 
than the breadth. Usually seen in pairs, closely resembling diplococci. 
When cultivated on potato it appears to be a micrococcus, but in the blood 
of infected animals and in bouillon cultures it is seen to be a short bacillus. 
Stains with difficulty with the usual aniline colors, but is readily stained 
by Ehrlich’s method or with Ziehl’s solution. 
Biological Characters.—An aérobic and facultative anaérobic, non- 
liquefying, non-motile bacillus. Spore formation not observed. Grows best 
