NOT DESCRIBED IN PREVIOUS SECTIONS. 559 
the spleen is enlarged, and hemorrhages in the serous membranes are usually 
seen, 
BACILLUS OF PURPURA HAMORRHAGICA OF KOLB. 
Obtained by Kolb (1891) from the various organs of three individuals: 
who died in from two to four days from attacks characterized by suddenly 
developed fever, purpura, and albuminous urine. 
Morphology.—Oval bacilli, usually in pairs, 0.8 to 1.5 long and 0.8 4 
broad, surrounded by a narrow capsule, which is only seen distinctly in 
preparations from the organs. 
Stains with the aniline colors, but not deeply, and still more feebly by 
Gram’s method. 
Biological Characters.—An aérobic and facultative anaérobic, non- 
liquefying non-motile bacillus. Does not form spores. Grows in the usual 
culture media at the room temperature. In gelatin. stick cultures, at the end 
of four days, a very small, thin, hyaline growth is seen about the point of 
inoculation. The development is more abundant along the line of puncture. 
Upon the surface of agar a thin layer is formed with smooth margins. 
Upon potato, at the end of three to four days, a whitish, moist, shining stripe 
is seen along the impfstrich which is about three millimetres broad. 
Pathogenesis.—Injections of 0.5 to 1 cubic centimetre of a bouillon 
culture into the abdominal eavity of rabbits cause symptoms of general in- 
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Fie. 157. Fia. 158. 
Fig. 157,—Bacillus gracilis cadaveris, from a gelatin culture. x 1,000.. From a photomicro- 
ph, (Sternberg.) ; 
Ee, 158.—Bacillus gracilis; colonies in gelatin roll tube, end of forty-eight hours. X 12. From 
a photograph. (Sternberg ) 
fection in the course of a few days, and not infrequently hemorrhagic ex- 
travasations are seen in the ear muscles. More than one cubic centimetre 
may cause death in from one to three days. At the autopsy hemorrhagic 
extravasations are found in the subcutaneous tissues and in the serous and 
mucous membranes. The blood has little disposition to coagulate; the 
bacillus may be recovered in pure cultures from the various organs. In 
guinea-pigs local ecchymoses are sometimes produced, otherwise not patho- 
genic for this animal. Pathogenic for mice, which die from general infec- 
tion, after being inoculated with a small quantity of a pure culture, in from 
two to three days; spleen enlarged; lymphatic glands often hemorrhagic. 
Not fatal to dogs, but animals which were inoculated with one cubic centi- 
metre of a bouillon culture and subsequently killed proved to have hemor- 
rhagic extravasations in the various organs. 
