NOT DESCRIBED IN PREVIOUS SECTIONS. 567 
_ _ Pathogenesis.—From 0.28 to 1 cubic centimetre of a bouillon culture 
injected into the pleural cavity of a rabbit caused a development of all of 
the symptoms of influenza (Brustseuche)—viz., elevation of temperature at 
the end of five or six hours, cough, nasal discharge, dyspnoea, and death— 
usually in from three to five days. The autopsy showed a distinct pleuro- 
pneumonia and a general blood infection by the bacillus in question. 
Injections into the circulation also give rise to the symptoms of influenza, 
including pneumonia, and to death at the end of from ten to fourteen days. 
Subcutaneous injections resulted in the development of an abscess and of ex- 
tensive necrosis of the tissues, but did not cause a general blood infection. 
Guinea-pigs were somewhat less susceptible than rabbits, but injections into 
the pleural cavity produced similar symptoms and death at a later date. 
White mice and house mice, as a result of intraperitoneal injections, died 
within two or three days from general blood infection. 
BACILLUS PISCICIDUS (Fischel and Enoch). 
Obtained by Fischel and Enoch (1892) from an infected carp. 
Morphology.—Bacilli solitary or in chains of four to five elements, 1.2 
to 3 long and 0.25 thick. Stains by the usual aniline colors and by 
Gram’s method. 
Biological Characters.—An aérobic and facultative anaérobic, non- 
motile, liquefying bacillus. Forms spores. In gelatin plates forms round 
colonies of a pale yellowish-brown color, having a slightly toothed border 
anda granular surface. At the end of twenty-four hours a narrow zone of 
liquefaction can be discerned around the colonies, and at the end of about 
ten days the gelatin is entirely liquefied. In gelatin stab cultures a scanty 
growth is seen along the line of inoculation at the end of twelve hours; the 
growth upon the surface is rapid, and liquefaction commences at the end of 
twenty-four hours. Upon agar, at 37° C. at the end of eighteen hours, a thin 
granular layer is seen, which consists of small, pale-gray colonies. In agar 
stick cultures a scanty growth occurs along the line of puncture, which does 
not increase after thirty-six hours. Upon the surface the growthis abundant, 
forming, at the end of five days a tolerably thick grayish-white layer. No 
growth occurs upon potato at the room temperature, but at 87° C. a tolera- 
bly thick, sticky layer of a grayish-white color is developed in three or four 
days. In bouillon, at 37° C., the medium is clouded at the end of twelve 
hours, and a thin pellicle is seen upon the surface at the end of thirty-six 
hours; this falls to the bottom when the tube is slightly agitated. At the 
end of four days development has ceased, and the bouillon is again transpar- 
ent, while a flocculent deposit is seen at the bottomof thetube. The bouillon 
gives off a penetrating odor, like that of burnt milk. Thesame odor is given 
off from cultures in milk, which is peptonized by the action of the bacillus. 
At the end of twenty days, at 37° C., the entire contents of the tube have be- 
come transparent. 
Pathogenesis.—Produces a fatal infectious disease in fish (‘gold carp”) 
when inoculated beneath the skin ; also pathogenic for mice and for guinea- 
pigs. 
BACILLUS PYOGENES FILIFORMIS (Flexner). 
Obtained by Flexner (1895) from the interior of the uterus and from an 
exudate in the pericardial and pleural cavities, of a rabbit which died on the 
fifth day after parturition. ; oe 
Morphology.—Pleomorphous cocci-like forms, short or long bacilli, and 
long threads are seen in cover slips prepared from the exudate. ‘‘Very few 
of the bacilli stain regularly ; for the most part brightly stained spots appear 
between stained areas. An outer membrane always stains, enclosing the 
