654 BACTERIA OF THE SURFACE OF THE BODY 
Bacillus typhi abdominalis, Spirillum cholere Asiatice. When to 
ten cubic centimetres of saliva, sterilized by filtration through porce- 
lain, the above-mentioned pathogenic bacteria were added in small 
numbers by means of a platinum needle carried over from a pure 
culture, no development occurred, and at the end of twenty-four 
hours the bacteria introduced were incapable of growth in a suitable 
medium. But when this amount of filtered saliva was inoculated 
with a large platinum loop—an dse—a certain number of the bacteria 
survived, and at the end of three or four days an abundant develop- 
opment occurred. At first, however, the number of living cells was 
considerably diminished. In saliva to which one ése of a culture of 
Staphylococcus aureus was added thirteen thousand eight hundred 
and forty colonies developed in a plate made immediately after inocu- 
lation, while a plate made at the end of twenty-four hours contained 
but one hundred and thirty-two colonies, and one at the end of forty- 
eight hours had but eight colonies. Subsequently multiplication 
occurred, and a plate made on the ninth day after inoculation con- 
tained so many colonies that they could not be counted. 
The diphtheria bacillus was not destroyed in filtered saliva, but 
did not multiply in it. On the other hand, it proved to be a very 
favorable medium for the development of Micrococcus pneumoniz 
croupose. 
Mucus from the surface of the meatus urinarius of man and 
woman, or from the vagina, will always be found to contain various 
bacteria ; but the bladder, the uterus, and Fallopian tubes in healthy 
individuals are free from microérganisms. 
Winter has isolated twenty-seven different species from vaginal 
and cervical mucus, and reports that he found Staphylococcus pyo- 
genes albus in one-half of the cases examined. <A streptococcus was 
also encountered which resembled Streptococcus pyogenes, although 
not positively identified with it. Samschin, on the other hand, failed 
to obtain the pus cocci in vaginal mucus from healthy women. 
Dénderlein, Von Ott, and others have carefully examined the 
lochial discharge with reference to the presence of bacteria. The 
first-named author found thatin healthy women the lochial discharge 
obtained from the uterus was free from germs, but when collected 
from the vagina various microdrganisms were obtained. In one case 
in which some fever existed Staphylococcus pyogenes aureus was 
found in the vagina, while the discharge from the uterus was free 
from germs. In five cases of puerperal fever Streptococcus pyogenes 
was obtained in the lochial discharge from the uterus. The results 
of Von Ott correspond with those of Dénderlein. Czerniewski, in 
the lochia of fifty-seven healthy women, found the Streptococcus 
pyogenes but once, while in the lochial discharge of fatal cases of 
puerperal fever it was always present. 
