CONTENTS. 



CHAPTEE I. 

 INTRODUCTION. 



PAGE 



Bacteria : their history and place in Nature — The abiogenesis and 

 biogenesis theories — Structure of micro-organisms — Types and 

 size of organisms — Method of reproduction — Rate of growth — 

 Movements of bacteria — Classification of micro-organisms — 

 Conditions and products of growth — Variation of bacteria — 

 Eesistance of bacteria to physical influences : light, heat, etc. — 

 Sterihsation — Action of cold, desiccation, electricity, chemical 

 agents - ... 1-27 



CHAPTEE II. 



BACTERIOLOGICAL AFFABATUS— FBEFABATION OF NUTRIENT 

 MEDIA, Etc. 



The apparatus used in bacteriological research — The microscope 

 — Hot air and steam sterilisers — High-pressure steam steri- 

 lisers — Intermittent sterilisation — Sterilisation by means of 

 chemical agents and filtration — Freezing and other microtomes 

 — Incubators, warm and cool — Centrifugal machines — Other 

 bacteriological apparatus — Nutrient media and their prepara- 

 tion - ... 28-63 



CHAPTEE III. 



METHODS OF BACTERIOLOGICAL STUDY— STAINING, Etc. 



The study of micro-organisms by means of pure cultures — 

 Gelatine plate cultures— Esmarch's roll culture— Streak, stab 

 and shake cultures — Culture of anaerobic bacteria — Hanging 

 drop culture — Permanent cultures — Indol reaction — Methods 

 of staining and mounting bacteria, their spores and flageUa — 

 The imbedding and cutting of sections of tissues — The staining 

 of micro-organisms in sections .... 64-101 



