DISEASES DUE TO PARASITIC FUNGI 299 



be stained witli eosin, and permanent preparations may be 

 put up in glycerine. 



Adamson {Glasgow Medical Journal, xlvi., 236, after 

 British Journal of Dermatology) recommends the following 

 method for permanently staining trichophyton tonsurans .- 

 (1) Soak the hair in a 5 to 10 per cent, solution of caustic 

 potash for ten to thirty minutes on a slide ; (2) wash in 15 

 per cent, alcohol in water ; (3) dry on a slide, and in the case 

 of scales fix by passing through the flame; (4) stain in 

 aniline gentian violet (made in the usual way, by adding a 

 few drops of saturated alcoholic solution of gentian violet to 

 aniline water) fifteen to sixty minutes ; (5) one to five 

 minutes in Gram's iodine solution ; (6) decolourise in aniline 

 oil two to three hours or longer ; (7) remove superfluous 

 aniline oil by blotting-paper, and mount in Canada balsam. 



The disease affects man, dogs, cats, cattle, and many 

 other animals. 



PROTOZOA IN DISEASE. 



A number of organisms have been noticed by various 

 observers in the blood of both man and the higher animals; 

 they occur associated with a number of diseases, but they 

 often occur in the healthy subject. These organisms belong 

 to the animal, and not the vegetable, kingdom. 



The Protozoa are unicellular bodies which can only live 

 in a moist or liquid medium, and in the absence of moist 

 nutrient material they become converted into round re- 

 sistant cysts. Protozoa may also possess a kind of larval 

 condition, consisting of small roundish or irregular masses 

 of protoplasm which move by means of projecting, limb- 

 like processes (pseudopodia), or in some cases by flagella. 

 They frequently lose their motility when they take up their 

 residence in other cells. The contents of the cysts separate 

 by division into particles known as sporocysts, the contents 



