444 APPLIED BACTERIOLOGY 



the colonies are usually brownish, the outer zones are light 

 yellowish-green. The colonies show radiate markings. 



Gelatine Streak. — Grows abundantly both on the surface 

 and ia the depth, and the whole tube assumes a green 

 fluorescent colour. The gelatine is not liquefied. 



Potatoes. — Produces a somewhat restricted growth, which 

 is at first reddish, but later becomes nut-brown in colour. 



Spirillum Finkler-Prior.— This organism was isolated by 

 Pinkler and Prior in 1884, from the stools of persons 

 suffering from cholera nostras. Microscopically, it is very 

 similar to the spirillum of Koch, but is distinguished from 

 it by its ability to grow on potato at room-temperature, 

 while cholera will only grow at blood-heat. It does not 

 produce the indol reaction in so short a time as the cholera 

 spirillum. Cultural characters : 



Gelatine PZaies.— Grows very rapidly in the form of 

 small white points, which under a lens appear to have 

 well-defined outlines, and are yellow or yellowish-brown in 

 colour. 



Gelatine rwies.— Liquefaction takes place at an early 

 date, and proceeds rapidly. The liquefaction occurs in 

 the form of a funnel-shaped tube, the liquefied gelatine 

 becoming very turbid. 



Agar-Agar.— A yellowish-white film is formed. 



Potatoes.— A yellowish- white layer is formed at the room- 

 temperature. 



Bacillus Fluorescens liquefaciens.— This organism occurs 

 m water, air, soil, etc., and occurs more frequently than 

 any other form. It forms short bacilli, about 1 to IS fj- 

 long by 0-5 fj. broad. It occurs chiefly in pairs ; is very 

 motile. Cultural characters : 



GelaUne Plates.— Forms small white dots; after about 

 forty-eight hours the gelatine becomes liquefied, and a 



