454 APPLIED BACTBEIOLOaY 



Potatoes. — Slow growth, producing a yellowish-white 

 expansion, which is restricted to the line of inoculation. 



Sarcina Lutea. — This organism is found in air, water, etc. 

 It forms large cocci, from 1-5 to 2-5 fi in diameter, and 

 arranged in twos, fours, and eights, in the usual packet- 

 like form. The organisms are non-motile, and are very 

 easily stained by the usual aniline dyes. Cultural char- 

 acters : 



Gelatine Plates. — Grows slowly, producing small round 

 yellowish colonies. 



Gelatine Tubes. — A slow-growing yellow expansion is 

 produced, made up of a number of raised protuberances. 

 The gelatine is slowly liquefied. 



AgaA--Agar. — A thick chrome-yellow growth spreads over 

 the surface of the medium. 



Potatoes. — Slow growth. The colonies are restricted to 

 the line of inoculation. 



Bacillus Subtilis {Hay bacillus). — This organism occurs 

 in hay, the air, water, the faeces, etc. It is about 6 /i long 

 by 2 /u. broad, about the same length, but somewhat 

 narrower than the anthrax bacillus. The organism grows 

 into long threads, and is very motile, having long flagella. 

 Forms ovoid spores about 1*2 /x long by 0*6 fi broad. These 

 spores are very resistant to heat ; they will bear exposure 

 to dry heat of 120° C. for one hour. The bacillus is strictly 

 aerobic. Cultural characters : 



Gelatine Plates. — The colonies become visible in about 

 two days, as small white dots in the depth, whereas on 

 the surface they show small grayish liquefied circles. 



Gelatine Tubes. — Forms a liquefied, funnel-shaped de- 

 pression, the lower part throwing out lateral feathery 

 extensions. The whole of the gelatine is soon liquefied 



