APPENDIX. 



THE PRESERVATION OF MATERIAL.* 



The method commonly used in the preparation of material for 

 dissecting purposes consists in first embalming the body with 

 suitable preserving fluids; afterwards filling the arteries with a 

 colored injection mass, so that they are more easily traced. The 

 objects served by embalming are: (i) preserving the body from 

 decomposition for a sufficient length of time to complete the 

 dissection ; (2) keeping the body as nearly intact as possible ; and 

 (3) having the organs in good condition for study. The point last 

 mentioned is an important one, since much depends on having the 

 parts of the animal in such condition that they are easily and 

 comfortably handled, and also easily observed. The desired results 

 are accomplished, first, by introducing the preserving fluid through 

 the bloodvessels, instead of by immersing the animal, as was 

 formerly the practice; secondly, by using in the preserving fluid 

 such materials as will leave the organs in a condition as near the 

 natural one as possible and at the same time keep them moist and 

 flexible throughout dissection 



A suitable fluid for the purpose is that recorhmended by Keillert 

 for the preservation of human subjects. The formula is as follows: 



Formalin 1.5 parts 



Carbolic Acid 2.5 " 



Glycerin 10. o " 



Water 86.0 " 



100. o 



*The methods here given apply only to the preservation of specimens for 

 ordinary dissection, either singly, or in numbers for a laboratory course ; with a few 

 observations on the difficulties which are likely to be experienced. Especially in 

 the matter of injections, the student who has acquired some knowledge of the 

 vascular system will be able to make complete injections of the portal system and 

 also satisfactory injections of the systemic veins, though the latter are somewhat 

 more difficult on account of the presence of valves in the vessels. Finer vascular 

 injections and injections of the lymphatic system according to the directions given 

 in the anatomical textbooks may also be suggested. 



fKeiller, W. "On the Preservation of Subjects, etc." {American Journal of 

 Anatomy), Vol. II., 1902-3. 



