26 Proceedings of the Royal Physical Society. 



This stock was allowed to die out naturally, but the hive had been removed 

 to another site, and the original stance disinfected. A small remnant was 

 found stiff and motionless on one comb, on 29tli February 1912. Thorough 

 disinfection of the site was effected by spraying crude carbolic acid, of such 

 strength that the worms came up and died, and the grass also was killed. 

 All inside fittings of the hive were burned, paraffin was also poured on the 

 hive site, and burned off 



Fourth Attack. Stock DJ/.. — This stock, a strong one, crowded with bees 

 and brood, along with two others, came from Exeter on 21st May 1912, 

 where as yet the disease was unknown. It was placed on the stance 

 of Dl, referred to above. Isle of Wight disease symptoms appeared on 

 28th May, exactly one week after the arrival of the stock upon the island, 

 and large numbers of crawling bees were observed in front of this hive on 

 every fine day. On 8th June, all the stores were exhausted and all the brood 

 was dead. Syrup was supplied, and the queen laid more eggs. On the 

 13th June there were still numerous bees, eggs, a few grubs, and food. At 

 this date samples were sent to Cambridge, and Dr Graham-Smith's report 

 upon them is as follows : — 



"The bees you sent all arrived dead. No spores were found by 

 microscopic examination, and it was impossible to say for certain whether 

 young stages of Nosema were present or not. I tried to infect some healthy 

 bees, but this experiment has turned out negative. 2/7/12." 



On the 17th bees were lying about in large numbers on the alighting 

 board and on the grass around, and dying off rapidly, 



On the 11th July, the remaining bees, along with queen, were sent to 

 Dr Graham-Smith, who kept them for some time. His report regarding 

 them is : — 



" The bees you sent have been doing moderately well, and nothing very 

 special has yet been found in those which have died." 



This must mean that Nosema was not found, yet the behaviour of the 

 bees, and the high mortality clearly indicated Isle of Wight disease. 



In the case of this stock no disinfecting measures were adopted, nor in 

 any subsequent instance in the Nicolson. Apiary, Stornoway. . 



Fifth Attack. One Stock {K.M.).— This stock arrived in Stornoway from 

 the South of England on 31st May 1912. Its ten combs were crowded on 

 11th June and a super was added. The super was quite full of bees on 

 22nd June, and some sections were ready for sealing on 13th July. Later a 



