44 Proceedings of the Eoyal Physical Society. 



Four samples of dead bees were taken. Two of these were from collections 

 of bees which had been infected by feeding with Nosema spores nearly three 

 months previously, and the remaining two consisted of bees from Isle of 

 Wight diseased stocks. 



(a) Pulp of twenty-six iVbsema-infected bees yielded, on examination, 

 numerous spores in the first and every succeeding drop of fluid taken 

 from the mass. 



(&) Pulp of a second (separate) lot of JVbs«ma-infected bees — about a 

 dozen bees. Eesult as in case of {a). 



(c) Pulp of bees, " crawlers," from two stocks — different apiaries on 

 Deeside — same stocks as were used for infection in second Shawbost 

 experiment (p. 54)— about twenty bees. Eesult ; No Nosema spores 

 observed after repeated examinations. 



{d) Pulp made of about two thousand bees from a stock which had died 

 out from Isle of Wight disease at- Banchory. The fluid, after 

 draining off from the solid residue, was tested extensively (twenty 

 separate examinations), and Nosema spores were not found. 



Other similar tests are given below: 



1. Thirty "crawlers" from DESl stock (p. 57) were picked up on 10th 

 September. They were allowed to die in a small cage, and on 14th were 

 pulped in a little water in the usual way. In this stock there had been found 

 previously an unidentified organism. The first field examined in the first 

 small drop contained numerous spores of this organism. Nosema spores were 

 not seen. 



2. Sample from Anguston, consisting of nineteen "crawlers," had their 

 abdomens pulped and examined for Nosema spores. Negative result. 



3. On 11th September 1915, visited Anguston and found nineteen of the 

 stocks had succumbed to Isle of Wight disease since last visit. Took a 

 sample of dead bees from the site of one of the dead stocks. 



On 20th September these bees, sixty in number, were pulped, and 

 examined. Nineteen samples were dealt with and no Nosema found. 



4. On 25th September 1915, seven bees which had died in transit from 

 Glenhouse, Stornoway, were pulped. Negative result as regards Nosema 

 spores. These were "crawlers" from the front of the hive, but we have no 

 other record of Isle of Wight disease in connection with this stock. Seven 

 live ones which survived the journey were similarly treated. Eesult : Negative 

 as regards Nosema. 



5. Six bees which remained of the lot of Carniolans (No. 7) from 

 Stornoway were pulped on 25th September. No Nosema was found. 



