50 Proceedings of the Eoyal Physical Society. 



On 7th October, it was found that sixteen bees of the infected lot had died. 

 These were pulped (abdomens) and examined for Msema spores. Free spores 

 in moderate numbers were found ; two or three in every field examined of 

 the first drop taken. One live bee examined gave a negative result. . In the 

 control, two bees had died. These were pulped (abdomens only), but no spores 

 were found. 



16th October 1915.— Five bees dead. These were pulped on 18tb, and 

 Nosema spores were found. Other three were examined, and spores found in 

 all. Other six were pulped, and spores were again found. All the control 

 bees had died at this date. 



From these results it seems probable that no infection had taken place. 



IV. .^^A January 1916. — On this date all the Desswood stocks were in 

 flight. The opportunity was taken to procure a number for experiment, and 

 samples were captured on the alighting board of the " Kelly " hive. These 

 were fed with candy, which on the same day was well smeared with pulped 

 matter from bees of the June experiments. This matter was found on 

 preliminary tests to contain Nosema spores in plenty. 



The bees were kept in a small wooden cage with glass cover. By the 6th 

 several had died. Mne were removed at this date, but of these, two were 

 dead shortly after caging, having probably been hurt. 



6th January 1916. — Two were allowed out of the cage, and these flew 

 quite strongly, and one of them defecated. 



8th January 1916. — Other six bees were found dead. One released 

 proved unable to fly, although it was quite active. When lifted it used its 

 sting. This bee had chyle-stomach, pale in colour, and colon dilated with a 

 pasty mass of pollen. Search in the dead bee and in the surviving one failed 

 to find any trace of Nosema spores. Another live bee very active was taken, 

 killed and examined. This bee had a pale chyle-stomach and inflated watery 

 colon. Numerous free spores were found in the latter, but a very thorough 

 search failed to discover any trace of infection in the chyle-stomach. 



mh Januarg 191 6. —On the 12th all the bees had died. These 

 (thirteen) were pulped, and examination showed free Nosema spores in 

 moderate numbers. 



V. ^th June.— Infected two boxes of "Kelly" bees with Nosema 

 material from No. 7 Stornoway hive. 



1st July.— Examined two bees which had died. Planonts seen in both 

 bees and intra-cellular forms in stained film. No free spores, seen. 



8th July,— On this date the chyle-stomach of a bee of this experiment, 

 taken alive, was found on examination to contain enormous numbers of 

 spores and planonts. Spore clusters common. 



