56 Proceedings of the Royal Physical Society. 



not occur in other two similar experiments, it is not clear that this was due 

 to the artificial infection. There were specially adverse circumstances in 

 the present case. In the other two instances of attempted infection, the bees 

 were maintained in good general external conditions. It should be noted 

 further that Nosema was not found at any time during the experiment. 



The foregoing experiments in artificial infection were made in Lewis upon 

 isolated stocks. The following were carried out in Aberdeen. 



No. 5. 60 DD. May 1916. 



This stock, which stood in an apiary in a district suffering badly 

 from Isle of Wight disease, was found, on 24th September 1914, to have 

 large numbers of bees on the alighting board, and groups of " crawlers " 

 on the grass around. The weather conditions were fine and sunny. The 

 same thing was observed on the following day, and the hive and bees were 

 removed to an observation area in Aberdeen. For a few days after this the 

 stock seemed to be doing well enough and a good deal of pollen was being 

 taken in. On fine sunny days, however, " crawling '' became very evident, 

 and on some occasions it was very extensive. As recorded elsewhere a 

 sample of the " crawling " bees was utilised in an infection experiment 

 (p. 53). 



On 7th October, which was a fairly good day with a little sunshine, 

 " crawling " was again in evidence, but pollen was still being gathered. 

 About this time it was reported that three other stocks from the same apiary 

 were suffering from the disease. All these eventually perished (p. 34). 



Up to 17th October, bees " crawled " every fine day, but the last of them 

 perished in the early winter, leaving abundance of stores in the hive. 



On 19th May 1915, a fresh stock arrived from the South of England, 

 Italian hybrids, fully covering ten combs crowded with brood. The queen 

 had apparently been recently lost, but there were numerous queen cells on 

 the combs. The new stock was put in the hive in which the above-mentioned 

 stock, 60 DD, had been housed. There was no disinfection of any kind, but 

 the dead bees and the old combs were removed. The honey was drained 

 from some of those old combs and fed to the new stock. 



On 3rd June, a swarm came off and was duly hived in a perfectly new 

 hive on frames with full sheets of foundation. This swarm was also fed on 

 honey drained from the old combs left by stock 60 DD. 



On the afternoon of the following day a second swarm came off and was 

 hived in a straw skep. This second swarm received none of the honey from 

 the old combs, but was fed on a little honey bought in the open market. 



