SCHEDULE FOR TWELVE LABORATORY 

 EXERCISES OF TWO-HOUR PERIODS 



Exercise I. Washing, plugging, and sterilizing glassware. 

 Exercise II. Making of media. 

 Exercise III. Technique of : 



Inoculation of media. 



Preparation of plates. 



Hanging drop. 



Use of microscope. 

 Exercise IV. Making and microscopical examination of stained 



preparations. 

 Exercise V. Observation of bacilli: 



Spore-bearing. 



B. suhtilis. 



B. chromogenic, etc. 

 Pathogenic : 



B. coli communis. 



B. typhosus. 



B. tuberculosis. 



B. diphtheria. 

 Demonstration of Widal's test. 

 Exercise VI. Observation of pathogenic micrococci : 



Staphylococci. 



Streptococci. 



Pneumococcus. 



Gonococcus. 

 Spirilla : 



S. cholerce. 



Treponema pallidum. 

 Exercise VII. Demonstration of anaerobic cultures. 

 Exercise VIII. Bacteriological examination of water; from five 

 or six sources and after sterilization. 



