APOPLECTIFORM SEPTICEMIA AND SLEEPING DISEASE 29 
pears ciliated. Similar but more profuse growth occurs on glycer- 
ine agar, and serum-gelatin-agar. 
In stab cultures on gelatin, growth is not observed until the 
fourth or fifth day. It consists of a finely granular line of minute 
spherical colonies, grayish white in color. The colonies have a 
fimbriated border and do not exceed a pinhead in size. The growth 
is not abundant nor characteristic and does not spread on the sur- 
face. No liquefaction occurs. Colonies on gelatin plates resemble 
those on agar, but are slightly more opalescent. 
Growth on blood serum appears within 24 hours in the form of 
small whitish colonies .6 to .8 mm. in diameter. Sometimes the 
color has a yellowish tint. The colonies do not coalesce. The water 
of condensation becomes turbid by reason of the presence of small 
gray clusters of the streptococci. 
Growth in milk occurs without causing visible change. In old 
cultures there is observed a solidification of the lower stratum, vis- 
ible when the tube is turned upside down. 
In litmus milk, the blue color is changed to a pale madder pink, 
indicating acid formation. No growth occurs on potato. No indol 
is formed. 
Bouillon containing one per cent of glucose, lactose and saccha- 
rose, in fermentation tubes, forms a favorable medium. Growth 
is more profuse than in plain bouillon and extends into the closed 
arm. Gas is not produced in these sugars, but acid formation oc- 
curs. Similar results are obtained with galactose, maltose and the 
alcohols mannite, dulcite and sorbite. 
No hemolytic effect occurs in Conradi-Drigalski plate cultures. 
Acid formation occurs on litmus-lactose-agar plates. 
Morbid anatomy. The skin displays hemorrhagic discolora- 
tion on the breast and neck, due to diffuse hemorrhages in the sub- 
cutaneous and muscular tissues. The abdominal cavity contains 
an abundance of sero-sanguinous exudate. Similar exudate some- 
times is present in the pericardial sac. The veins of the mesentery 
are engorged. The liver is greatly enlarged, paler in color than 
normal and the entire surface is covered with a semi-organized 
plastic exudate. The spleen may be similarly swollen. The gall 
bladder is distended with bile, while the kidneys are hyperemic 
and swollen. The intestines, especially the duodenum, are observed 
from the peritoneal surface to be congested in patches. On opening 
the intestines, the mucosa corresponding to the discolored areas 
is considerably swollen. The contents of the intestine consist of 
