FOWL CHOLERA 35 
dulate border. The condensation water becomes decidedly turbid. After 
the first week of growth the liquid clears somewhat with the deposition of 
a viscous sediment. No pellicle has been observed on the condensation 
water. 
“ Glycerine Agar. Growth upon this medium presents no features dis- 
tinguishable from that upon agar slant. 
“Gelatin. Surface colonies, after about two weeks’ growth at room tem- 
perature, are round, vitreous masses, with entire border and smooth shiny 
surface. A large proportion of the colonies are raised, forming a conical 
mass not exceeding 1 mm. in diameter. Such colonies appear highly re- 
fractive by directly transmitted light. Under a two-thirds objective the 
colonies have a finely granular appearance and show concentric circular 
markings. Sub-surface colonies are lenticular in shape and granular. 
“ After three days’ growth under similar conditions the growth in gelatin 
stab cultures is noticeable as a mass of closely aggregated colonies near 
the surface. After about two weeks the surface growth appears as a round, 
thin gray mass, with contoured surface and undulate border. At the same 
time the growth along the whole length of the path of the inoculating 
needle appears as a mass of closely aggregated distinct colonies. 
“ Potato. Implantations on this medium have not resulted in visible 
growth. 
“ Alkaline Bouillon. After forty-eight hours at 37.5° C. the fluid becomes 
slightly clouded and does not clear up on standing, even after four months. 
In cultures two or three days old no sediment is deposited, but in older 
cultures a viscous sediment accumulates. The reaction is alkaline to 
litmus and markedly so in old cultures. No pellicle is formed, but occa- 
sionally a circular bluish band of growth adheres to the tube at the level 
of the surface of the fluid. 
“ Sugar-free Bouillon. Growth is similar to that in alkaline bouillon. 
“Acid Bouillon. The growth is similar in appearance to that in alkaline, 
except that the turbidity is less marked and no accumulations have been 
noticed at either surface or bottom of liquid. Reaction becomes alkaline 
in old cultures. 
“ Milk. No change occurs in this medium during the length of time that 
it is ordinarily kept under observation. 
“ Fermentation Tubes. (1) One per cent glucose bouillon: The liquid 
throughout the tube becomes uniformly slightly clouded in twenty-four 
hours at 37.5°C., and remains so. The reaction becomes acid in two days. 
No gas is formed. A slight amount of viscous sediment collects. 
“ (2) One per cent lactose bouillon: The character of growth is similar 
to that in glucose. The reaction remains alkaline. 
“ (3) One per cent saccharose bouillon: The growth is similar to that of 
the two preceding. The reaction becomes acid in two days, but eventually 
becomes alkaline in cultures several weeks old. 
“Dog Blood Serum. After twenty-four hours at 37.5° C. the path of the 
needle is occupied by a smooth, shiny, raised growth of a color determined 
by that of the serum. The condensation water is markedly turbid. Six 
days later the growth, as well as the surface of a pellicle on the condensa- 
tion water, has a coppery lustre. 
