BACILLARY WHITE DIARRHEA 79 
acid is added in sufficient quantity to form a .5 per cent solution 
for preserving purposes. In the earlier experiments of the authors, 
the product was passed through a Berkefeld filter and concentrated 
to one-tenth of its original volume. Later it was found that the 
original sterilized product concentrated to % its volume gave as 
satisfactory results. 
The material is injected into either wattle of the fowl in the 
amount of approximately .2 
e.c. The injection is made 
with a hypodermic syringe 
graduated in tenths of a c.c., 
and a needle of fine caliber 
is used to avoid causing 
traumatic swellings. The 
injection is made at a point 
near the lower border of the 
wattle. Where the flock is ; 
apparently free ob inite- Pr sting positive roustion to tho intraden 
tious disease, no disinfect- mal test for B. pullorum infection. 
ants are employed at the (Original) 
point of injection since the natural resistance of birds to ordinary 
wound infection is sufficient to guard against infection. In the ab- 
sence of a graduated syringe, the amount to be injected may be 
gauged by injecting enough to cause a perceptible swelling at the 
point of inoculation. During the first three hours after injection 
the wattle shows an edematous condition which rapidly disappears in 
non-reacting birds. Readings are taken at twenty-four to thirty 
hours after injection. The wattle may be swollen to several times 
its natural thickness and may be readily observed at a distance, or 
the swelling may be so slight that palpation, or comparison with the 
uninjected wattle will be necessary. Any swelling other than that 
due to traumatism as a result of faulty technique, should be consid- 
ered significant. 
REFERENCES 
1. Bushnell and Maurer. The use of milk cultures of B. bulgaricus in 
the prevention and treatment of bacillary white diarrhea of young chicks. 
Am. Vet. Rev., Vol. 44, 1913, p. 195. 
9. Gage and Martin. Notes on the histo-pathology of the intestines in 
young chicks infected with bacterium pullorum. J. Med. Research, Vol. 
84, 1916, p. 149. 
3. Gage and Hyland. On the diagnosis of infection with bacterium 
