INFECTIOUS DISEASES OF CANARY BIRDS 207 
time there has formed a very shiny, thin layer, the border of which 
appears finely toothed. 
The organism remains living on agar for six weeks without trans- 
ferring and on blood serum for eight weeks. 
On agar plates after fourteen hours at incubator temperature 
there are formed sharply defined, gray-white colonies about the size 
of a poppy seed which lie partly on the surface and partly within the 
medium. ‘They do not change in appearance after a longer time. 
On gelatin plates one observes with the unaided eye, after forty 
hours, fine gray-white points. By reflected light under the micro- 
scope with low magnification colonies are seen to have a brownish 
yellow color, have sharp borders and are circular or oval in form. 
In the center of these are a number of dark brown granules and on 
this account the center appears darker than the periphery. After 
two days a bright, transparent area forms about each colony and 
after three days one observes distinct liquefaction. This advances 
until the whole plate is liquefied after five days, reckoned from the 
time that the culture was planted. Similar growth is observed on 
one per cent glucose gelatin plates. 
On slanted gelatin a distinct liquefaction occurs along the line of 
inoculation after forty-eight hours. On the bottom of the tube there 
is observed a large amount of cloudy fluid which has run duwn from 
the surface of the gelatin leaving a trough-like depression. In 
gelatin stab cultures after forty-eight hours punctiform colonies are 
observed along the line of the stab. There is a depression on the 
surface the size of a pinhead, which represents the beginning of 
liquefaction. 
The liquefaction progresses and in about fourteen days the whole 
of the medium becomes liquefied. Then there is observed a gray- 
white viscid mass in the bottom of the test tube which when shaken, 
ascends and forms a dense cloudiness. The organism remains alive 
on gelatin without transfer for about six weeks. 
In bouillon in the incubator after fourteen hours, the organism 
causes a uniform clouding and on shaking, a viscid sediment rises. 
The clouding continues till the third day and the sediment increases 
at the same time. After five days the upper part of the bouillon be- 
comes somewhat more clear and after fourteen days it becomes en- 
tirely clear. In the bottom of the test tube at this time there is an 
accumulation of brownish gray, thick sediment which on whirling 
the tube appears granular and stringy. No pellicile forms on the 
surface of the bouillon. Similar growth is observed in glycerine 
