CONTENTS. 



OUTLINE OP METHODS OF WORK. 



General Remarks 



The Disease 



Previous Literature 



Geographical Distribution 



Signs of Disease 



Pathological Histology 



Direct-infection Expernnents 



The Organism 



Pathogenesis 



Rules of Proof 



Morphology 



Size, Shape, etc 



Capsules 



Flagella 



Spores — Endospores, Arthrospores 



Cell-unions — Zoogloeae, Chains, Filaments. . 



Livolution-forms 



General Comment 



Physiology 



Motility 



Growth 



Chemotropism 



Reaction to Stains 



iCulture-media 



Nutrient Gelatin 



Nutrient Agar 



Silicate-Jelly 



Solid Vegetable Substances 



Plant Juices (with and without the addi- 

 tion of water) 



Animal Fluids 



Beef-broth 



Milk 



Litmus Milk 



Rice cooked in Milk 



Loeffler's Solidified Blood-serum 



Egg-albumen 



Egg-yolk 



Synthetic Media and Other Special Media. 



Relation to Free Oxj-gen 



Surface and Deep Growths 



Fermentation-tubes 



Growth in Hydrogen, in Carbon Dio.xide 



in Vacuo, and in 'Nitrogen 



Luminosity 



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54 

 60 



Page. 



The Organism — Continued. 

 Physiology — Continued. 



Fermentation-products 60 



Alkalies (Ammonia, Amins, etc. ) 61 



Reducing Powers 62 



1 lydrogen Sulphide 62 



Mercaptan and Other Odors 62 



Tndol, Phenol, Leucin, Tyrosin, etc 62 



Reduction of Nitrates, etc 63 



Fixation of Free Nitrogen, etc 64 



Assimilation of Carbon Dioxide 64 



Pigments 64 



Crj'stals 66 



Enzymes 66 



Sensitiveness to Plant Acids 69 



Sensitiveness to Alkalies 69 



Effect of Desiccation 70 



Effect of Direct Sunlight 71 



Vitality on Various Media 72 



Mi.xed Cultures and Mi.xed Infections 72 



Reaction to Antiseptics and Germicides 74 



Thermal Relations : Maximum, Minimum, 

 and Optimum Temperatures for 

 Growth ; Thermal Death-point ; Eff'ect 



of Freezing 75 



Other Host-plants 87 



Pathogenic or Non-pathngenic to Animals 



Economic Aspects 



Losses 



88 

 90 

 90 

 Natural Methods of Infection 91 



93 

 93 

 94 



94 

 94 

 96 

 97 



Conditions favoring Spread of the Disease. .. . 



Methods of Prevention 



General Considerations 



Location of the Laboratory 



Equipment of the Laboratory 



Care of the Lalioratory 



Preparation and Care of Culture-media 



The Cleaning and Sterilization of Glassware 



and Instruments 100 



Making and Transference of Pure Cultures. . . 103 

 The Final Disposal of Infectious Material.. . 106 



Methods of Inoculation 108 



The Keeping of Records 109 



The Making of Collections 117 



Distilled Water 124 



