FORMUI^.-E. 



197 



Synthetic Culture Media. 



I'astciii-'s Culture Fluid. 



i'Vnimonium tartrate 10 



Ashes of yeast 10 



Rock candy 100 



Distilled water 1,000 



Dissolve cold. 



Nacgcli's Nuliicitl Solutiou. 



Calcium chloride o.l 



Magnesium sulphate .2 



Dipotassium phosphate i.o 



Ammonium tartrate lo.o 



Distilled water 1,000.0 



Colin's Nutricul Solution. 



Distilled water 1,000,0 



Acid potassium phosphate 5.0 



)I\ra,L;ncsium sulphate 5.0 



Neutral ammonium tartrate 10 



Potassium chloride 0.5 



(Dc Bary, p. 86, Vorles. ii. Bact., 2 Auflage.) 

 Raulin's Culture-Fluid. 



Distilled water 1,500.00 



Granulated cane sugar 70.00 



Tartaric acid 4.00 



Ammonium nitrate 4.00 



Ammonium phosjihate .60 



Potassium carbonate .60 



.Magnesium carhonate .40 



Ammonium sulpliate .25 



Zinc sulphate .07 



Ferrous sulphate .07 



Potassium silicate .07 



Prazuioivski's Culture-Fluid. 



Dipotassium phosphate 5.0 



IMagne^ium sulphate 5.0 



Ammonmm carbonate 5.0 



Calcium chloride .5 



Distilled water 1,000.0 



Dissolve cold. Any desired sugar may be 

 added for the carbon food. 



.-Idolf Mayer's Culture-Fluid. 

 (Unters. ii. d. ale. Gahr., 1870.) 



Magnesium sulphate lo.o 



Ammonium nitrate 15-0 



Tri-basic calcium phosphate .1 



Potassium phosphate lo.o 



Distilled water 1,000.0 



Dissolve cold and add sugar. Add sodium 

 chloride (3 per cent) if it is to be nsed for 

 luminous bacteria, and an excess of pure car- 

 bonate of lime if acid-forming bacteria are to be 

 grown. 



Uschiusky's Solution. 



Distilled water 1,000 



Glycerin 30 to 40 



•Sodium chloride 5 to 7 



Calcium chloride 0.1 



Magnesium sulphate 0,3 to 0.4 



Dipotassium phosphate 2 to 2 5 



Ammonium lactate 6 to 7 



Sodium asparaginate 3 to 4 



Modified Useliinsky's .Solution. 

 The modified Uschinsky recommended b_\' the 

 writer for use with starch jelly is made as fol- 

 lows : 



Distilled water 1,000.000 



Ammonium lactate 5.000 



Sodium aspara.ginate 2 500 



Sodium sulphate 2.500 



Sodium chloride 2 500 



Dipotassium phosphate 2.500 



Calcium chloride .010 



Magnesium sulphate .010 



Fraeiikel and Voges' S ilution. 

 (Hygienische Rundschau, Bd. IV, 1894, p. 769.) 



Water 1,000 



Bodium chloride 5 



Dipotassium phosphate'"' 2 



Ammonium lactate 6 



Sodium asparaginate 4 



This paper also discusses Uschiusky's solution. 

 F'ernii's Culture-I'luid. 



Distilled water 1,000,0 



^lagnesium sulphate .2 



Acid potassium phosph.ate i.o 



Ammonium phosphate 10. o 



Glycerin 45.0 



This may be added ti 1 agar in place of pepton- 

 ized beef-broth (De Schweinitz) or to silicate 

 jelly, in which case the vi'lume of water must 

 be reduced (see Silicate Jehy, p. 36). 



Moore's Culture-Medium for Le.s^uuiiiious Root- 



tuberele Baeilli. 



(For Held use.) 



The dried cnlture (on cotton) is thrown into 



clean water containing: Cane-sugar, 1; c. p 



monopotassium phosphate, o.i; e. p. magnesium 



sulphate, o.oi per cent. After 24 hours add 



^'lerck's pure dibasic ammonium phosphate to 



amount of 0.5 per cent. Seeds are drenched 



with this fluid at end of another day, dried in 



shade, and planted. 



*Leliiiianu and Neumann recorameud neutral commercial sodium phospliate [t. <■. p. 29.) 



