222 



BACTERIA IN RELATION TO PLANT DISEASES. 



(Vii). PkECL, FkiTZ. Ueber eine neue Karbolmethy- 

 leiib'lan-Method. Centralb. f. Bakt., x Bd., 

 1X91, pp. K26-820. 



Prcgl finds the foUowiiiK mctliort of staining sections 

 on slides satisf.'Klnry : ■' Hxposnre for onedialf to one 

 niinnte to carbol methylen Ijlne, sometimes with the aid 

 of heat; second, tirief washing in water; third, lileaching 

 in 50 per centalcohol until the section has become pale 

 blue passing into .greenish ; fourth, removal of the water 

 by means of absolute alcohol; fifth, clearing iu xylol ; 

 sixth, enclosure in balsam." 



Kuehne's carbol methylen Idiie is made as follows ; i 5 

 grins, methylen blue, 10 grms. absolute alcohol, 100 grams 5 

 percent carbolic-acid water. The alcohol is poured over 

 the methylen blue; the carbolated water is then addedand 

 rubbed up thoroughly wdth it. If only a small quantity 

 is needed from time to time, it is better to make it up in 

 less amounts, as, with time, the staining po-iver of the 

 solution decreases. 



{''j2). KuEHNE, If. Das MalacbitR-rim als Aii.szieh- 

 iingsfarbc. Centralb. f. Bakt, xi Bd., 1S92, 

 PP- 75'>7S8. 



Author finds raalachit green dissolved in anilin oil very 

 useful for treatment of sections stained in carbol-fuch- 

 sin, etc. The l)acteria stand out distinctly deep red on a 

 bluish background. See paper for details. 



('03). XicoLLE, ET CanT.\cuzenE, J. Proprietes 

 colorantes de rox3'chloriire de ruthenium 

 ainmoniacak Ann. de rin.S't. Pasteur, T. 

 vti., pp. 33i-.«4- , ^ .,. 



('9S). Unn.v, p. G. Ueber \'erwenddng von Anihn- 

 mischungcn zur tinktoriellen Isolierung von 

 Gevvebselementen. Monatsbefte f. prakt. 

 Dermatologie, Bd. xxr, 1895. Rev. in Cen- 

 tralb. f. Eakt., I Abt , XX Ed , 1S96, p. 406. 



(95). NicOLT.E, M. Pratique des colorations micro- 

 biennes, Methode de Grant modifiee et 

 met.hode directe. Ann. de ITnst. Pasteur, 

 T. IX, 181)5, No. 9. Rev. in Centralb. f. 

 Bakt., XVIII Bd., 1895, p. 552. 

 Carbol violet is substituted for anilin violet. Excess of 

 color is removed withalcohol-aceton. 



I '07). Semenowicz, W'., und ^Iarztnowsky, E. 

 Ueber ein besonderes Vcrfabrcn zur Far- 

 bung der Bakterien im lJeck.glaspraparate 

 iund in Scbnitten. Centralb. f. Bakt., xxi 

 Bd., 1897, PP 874-876. 



Coo). Dkever, Georges. Bakterienfarbung in gleich- 

 zeitig nacb van Gieson's Methode behandel- 

 ten Schiiittcn. Centralb. f. Bakt., I Abt., 

 XXVII Bd„ 1900, pp. 534-535- 



Can be used for differential staining ""'f animal tissues 

 with bacteria in them, provided the latter are such as 

 stain b3' Grain's method. 



Coo). 1,1; Dou.x. Ecmcrkungcn zu dem Artikel des 

 llerrn M. Dor.iet : ".'V new stain for Bacillus 

 tuberculosis" Centralb. f. Bakt., i Abt., 

 xxvii Bd , 1900, p. fii6. 



Dorsett recommended Sudan III for staining the B 

 tuberculosis. I,e Iloux says he has tried this repeatedly 

 with entirely negative results, 



('02). Levinson, Ja. B. Ueber Fiirbnng des Fettes 

 und der felligeu Tiegineralion der gcforra- 

 ten Elemciite in flli^sigen und hallifliissigen 

 Mcdicn init Sudrm III Wratscli. St. 

 Peter.sberg, 1902, Ed. i, pp. i,2oS-t,209 

 (Russian.) 



('02). GrimmE, Ar.~^oed. T)ii- wicbti.gsten Mclhoden 

 der Bakterienfarbung in ibrcr Wirkung auf 

 die IMcinbran, den I'n iti:i|il:isLrn iiiid die 

 iMnsebliis^c der r.,ak'lerienzelle Ceiilriilli. f. 

 P.akt., Abt. T, jbl ,xx\ri, Originale, 1902, pp 

 1-16, PP 81-90, p|j. i6i-i8n, |jp 241-255, pp. 

 .121-327, wilh 2 plales. Eibling of 78 titles. 



XV. Morphological and Physiological Changes Due 

 to Changed Environment. 



('87). ScuoriELius. Uutersuchungcn fiber den 

 Microc. prndigiosus. Festschr. f. KoUiker, 

 I^eipzig, 1887. Not seen. 



This author obtained pigmented and non-pigmented 

 races of Bacillus prodigiosus, 



('89). Behrinc. IV-itrage zur Aetiologie des Milz- 

 brandes. vi. Ueber asporogenen Milzbrand. 

 Zeitschr. f. Ilyg , iSSg, Ed. vii, pp. 171-176. 



Author describes method of obtaining Aplanobacter 

 (Bacillus) anthracis without spores. 



('90). SlIITH. See VI. 



('90). Riitix, E. Baoteridie cbarbonneuse asporogene. 

 Ann. de I'lnst. Pasteur, T. iv, i8go, pp. 25-34. 



(92). Nencki, M. Ueber IMischkuituren. Centralb. 

 f. Bakt., XI Bd., 1S92, pp. 225-228. 



('92). Adajii, J. G. On the variability of bacteria 

 and the development of races. Med, Chron- 

 icle, vol. XVI, 1892, No. 6, pp. 366-3S9. Also 

 a reprint from Med. Chronicle, Sept., 1892, 

 8vo., 26 PP , Manchester (John Hevwood), 

 1892. 



(92). Ciiarrin, a., et Phisaeix. Abolition nersis- 

 tante de la fonction chroniogene du Bacillus 

 pyocyaneus — C. R. des se. de I'Acad. des 

 Sci., Paris, 1892. T. cxiv, pp. 1,565-1,568. 



('93). Sander. Ueber das Wachstum von Tubor- 

 kclbaciUen atif pflanzlichen Naht-boden. 

 Arch f. T-Iyg., Bd xvi. Heft 3, 1893, pp. 

 238-311. 



At ,sS^-,^9^ C. (not at 22-^V- C), this author induced the 

 organism of tuberculosis to .grow on a variety of vegeta- 

 ble substances, i. e., potato, carrot, kohlrabi, macaroni, 

 etc. All were cooked. 



('93). SleixEY, G. Suggestion as to a possible source 

 of the energy required for the life of bacilli, 

 and as to the cause of their small size. 

 Proc. Roy. Soc, Dublin. Vol. viii, Pt. i, 

 pp. 154-156. Dublin, 1893. 



V'icws of a jjliysici.^t. 



(94). SuRMONT, I-d., ET Arnould, E. Recherclies 

 sur la production du bacille du charbon 

 asporogene. Ann. de I'liist. Pasteur, T. viii, 

 1S94, pp. 817-832. 



('94). Ury, Jakob. Ueber die Schwankungcn des 

 Bacterium coli commune in morpbologis- 

 cher und kulturcller Be/iehung. ( Inang 

 Diss.) 8\-o , 47 pp., Strassburg i. E,, 1894. 

 Rev. m Centralb. f. Eakt., Ed. xvi. 1894, pp, 

 579-5S1. 



('94). S.MITH, TiiEOBAED. ]\'Iodification, temporary 

 and permanent, of the physiological char- 

 acters of b;icteria in mi.xed cultures. Trans. 

 Assoc. yVnicr. Physicians, Phila., 1894, pp. 

 S5-109. 



('94). DiEt'DONNi;, A. Beitrage zur Kcnntnis der 

 .Vnpassuugslahi.gkeit der Bakterien an ur- 

 sprunglich ungunstige Temperaturverhalt- 

 nisse. Arbeiten aus dem kaiserl. Gesund- 

 beitsamle, Bd. ix, 1894, pp. 492-508, Berlin, 

 1894 (■Julius Springer). ,Vlso a separate 

 Rev. in t'entralb. f. Bakt., Bd. xvi, 1S04, pp 

 9(15-967. 



('05). D wENEORT, C. E., .\Nn C-VSTEE, \A', E. On the 

 :icclim;ilizatiou of organisms to high tem- 

 peratures '\rchiv f. I'^ntwickelungsme- 

 cbaiiik der Orgaiiisnien, Ed. 11, TTeft 2, 1895, 

 |i|). 227-210 Bihlingrapln- iif 3 pages, 

 (irg.anisms become aecliiuaLi/ed loliigh temperature by 

 losing w-ater from their protoplaMii. 



