230 



BACTERIA IN RELATION TO PLANT DISEASES. 



('9S). MackEnsie, J. J. Opening discnssion on 

 "W'.hat new methods can ibe suggested fi">r 

 the separation of bacteria into groups, and 

 for the identification of species." Jour. Am. 

 Pub. Health Asso., Oct., 1895, Ann. vol. xx, 

 pp 419-4,^1- 

 Notes oil synthetic media. 



(97). Stoddart. See XVI. 



('97). IIlSS. See XVI. 



CgS). Houston, A. C. Note on four micro-organ- 

 isms isnlated from the mud of the river 

 Thames, which resemble Bacillus typhosus 

 CentraWi. f. Bakt., xxiv Ed., 189S, pp. 518- 

 525, colored diagram. 



(■98). Fermi. See xxxvi 



('98). RothbeegEr, C. Jueius. Differential diagnos- 

 tische Untersuchungen mit gefarbten Nahr- 

 .briden. Centralb. f. B.akt., xxiv Bd., 189S, 

 PP^ 513-.S1S. 



with neutral red (Toluidin red) in agar B. coli is said 

 to cause a clearing of the color and a verj' decided fluor- 

 escence. B. typhi leaves the medium unchanged. The 

 best method is said to be to add 3 to 4 drops of a concen- 

 trated water solution to 10 cc. of fluid agar, and then ' .j 

 cc. of a 24-hour old bouillon culture. In a.gar stained 

 with safraniu, B. coli reduces the color; B. typhi does 

 not. Dead cultures of B. coli (cultures heated 2 hours at 

 78° C.) did not produce these changes. 



('98). Pacinotti, G, and Muniecki, J. L'albume 

 d'uovo colorito in verde-cupo dal caffe 

 ■crudo, come mezzo diagnostico di sviluppi 

 ibatterici. Gazz. degli ospedali e della 

 oliniche, 1898, No. 31. Rev. in Centralb. f. 

 Bakit., XXV Bd., 1899, p. 257. 



('99.) RotheErger, C. Julius. Differential diagnos- 

 tische Untersuchungen mit gefarbten Nahr- 

 bodcn. II, Mitteilung. Centralb. f. Bakt., 

 xxv Bd., 1899, pp. 15-17 and 69-75. 

 Author tested the effect of various bacteria ou 35 auilin 

 dyes. 13 of which proved useful in agar media. The loss 

 of color in methylen blue, safranin, Toluidin blue, Or- 

 seille extract and indigo carmin is due to reduction pro- 

 cesses. In case of the indigo carmin, the blue was first 

 changed to dark green. 



(00). SchEFFlER, VV. Das Neutralrot als Hilfsmittel 

 zur Diagnose des Bacterium coli. Centralb. 

 f. Bakt., xxviii Bd., 1900, pp. 199-205. 



Author says B. coli in neutral red, grape sugar agar 

 gives regularly in 24 to 48 hours a beautiful green fluor- 

 escence. He uses : fluid agar, 100 cc, grape sugar, 0.3 

 gram ; concentrated watery solution of neutral red, icc. 



('01). HoELSCHER, AV.M.TER. Ueber die Differenz der 

 histologischen Wirkung von Tuberkel- 

 baciUen und anderen diesen ahnlichen 

 saurefesten Bacillen (Grasbacilltis 11 Moel- 

 ler, Butterbacillus Petri-Rabinowitsch, 

 Thimotheebacilkis MoUer). Mtindiener 

 med. Wochenschr., Bd. XEViii, 1901, pp. 

 1,48,3-1,484. 



('oj). Hiss, Philijp IL\nson, Jr. New and simple 

 media for the differentiation nf the colonies 

 of typhoid, colon, and allied bacilli. The 

 Journal of Medical Research, June, 1902, 

 vol. VIII, pp. 14S-167. Also a separate. 2 

 iplatcs. 



Author describes a simple solid medium in which 

 Vjuried colonies of the typhoid liacillus send out thread- 

 formed radiations, while those of B. coli, etc., do not 

 bear any fringing tlirea<ls This medium, used in I'etri- 

 dish poured plates, consists of distilled water 1,000, agar 

 15, gelatin 15, dextrose 10, I.iebig's extract meat ,5, sodium 

 chloride 5. 



('02). FiTz Geraed, MarEl Purefoy, and Dreyer, 

 Georges. The unreliability of the neutral 

 red method, as generally employed for the 

 differentiation of B. typhosus and B coli. 

 Festsksrift ved Indvielsen af Statens Serum- 

 institut, fvjobenhavn, 1902, pp. 1-39. 



('02). Buxton, B. IT. A comparative study of the 

 bacilli intermediate between B. coli com- 

 munis and B. typhosus. Jour. Med. Res. 

 Ill (n. s.), 'Pp. 201-230, 3 plates. 



Bibliography of 16 titles. 



('03). Gage, Stephen DeM., and Pheeps, Earle B. 

 Notes on B. coli and allied forms, with 

 special reference to the neutral-red reaction. 

 Proceedings of the Thirtieth Annual Meet- 

 ing, American Public Health Assn., New 

 Orleans, La., Dec, 1902. Pub. Columbus, 

 Ohio, 1903, vol XXVIII, pp. 402-412. Also a 

 separate, pp. 11. 



('03). ScHEiEDER. Zum Nachweis der Typhusbak- 

 terien im Wasser. Zeitschr. f. Ilyg., Ed. 

 XLII. 1903, pp. 317-326. 

 Describes differential methods. 



('04). Stokes, AA'ieeiam Royal. A simple test fiir 

 the routine detection of the colnii bacillus in 

 drinking water. Jour, of Infectious Dis- 

 eases, vol. r, 1904, pp. 341-347. I plate. 



Neutral red reaction. 



XIX. Aerobism, Anaerobism. 



(See also various citations under XX.) 



('61). Pasteur, Louis. Animalcules infusoires 

 vivant sans gaz o.xygene fibre et determinant 

 des fermentations. — ^C. R. des se. de I'Acad. 

 des set, Paris, 1861, T. lii, pp. 344-347. 

 ('63). Pasteur, Louis. Nouvel e.xcmple de fermen- 

 tation determinee par des animalcules in- 

 fusoires pouvant vivre sans oxygene libre, 

 et en dehors de tout contact avec I'air de 

 ratmosphere. — C. R., des se. de I'Acad. des 

 sci., Paris, 1863, T. LVi, pp. 416-421. 

 ('63). Pasteur, Louis. Recherchcs sur la putrefac- 

 tion. C. R. des se\ de I'Acad. des sci., Paris, 

 1863, T. LVi, pp. 1,189-1,194. 

 " Je propose avec toute sorte de scrupules ces mots 

 uouveaux aerobics (tl anaerobes pour indifinerl'existeuce 

 de deux classes d'etres iuferieures, les iiiis incapalile de 

 vivre en dehors de la pr^^sence du gaz o.xygene libre, les 

 autres pouvant se multiplier ;t I'infini en dehors du con- 

 tact de ce gaz." 



('80). BuchnER. LTeher die Lcbensfahigkeit der 

 Spaltpilze bei fehlenden Sauerstoff, iSSo. 

 Not seen. 



!'85). IIicssE. W. und R. Ueber Zuchtung der 



Bacillen des maligneii Oedems. Deutsche 



med, Woch en sell rift, 1885, 11 Jahrg., pp. 



214-215. 



Describes a method of cultivating anaerobic organisms 



by sowin,g them in deep masses of solid media. 



(,'86). LiBORius, Paul. Beitriige zur Kentniss d. 

 SauerstofThediirfnisses der Baktcrien. 

 Zeitschr. f. IT_\-g., Bd. i, 1SS6, pp. 115-177. 



According to review by Zimmerman in Bot. Centralb. 

 Bd. xx\'Jli, iSsi,, p. i,|8, author found but little more oxy- 

 gen in layers oi agar buried 3 cm. and o\cr uudel" addi- 

 tional agar than in vessels in which air was expelled by 

 hydrogen, etc. He used " Indigotinloesung " as a test. 



