62 s 



September 14, at 4 p. m., Cruz complained of feeling chilly and 

 had frontal headache. The chilly sensation lasted for several hours 

 and the headache became so severe that he was given ice caps to his 

 forehead for the relief of this symptom. The conjunctivse became 

 injected ; the. gums turgid and red. 



The case continued clinically with typical symptoms of yellow 

 fever. 



• On September 15, at § a. m., there was a slight , icterus of the 

 eyes ; at 10 a. m. there was marked vomiting ; at 7 p. m. the gums were 

 yery much swollen. Urine contained no albumin. 



September 16: Eyes injected and yellowish, skin jaundiced; no 

 albumin. 



On September 17, the third day of the disease, albumin first 

 appeared in the urine and was found daily until September 25, after 

 which examination was discontinued. 



On September 15, at 8 a. m., 16 hours after the onset of the disease 

 80 cc. of blood were drawn into a sterile flask by means of an aspirat- 

 ing needle from the median basilic vein of his left arm. 



The flask was set aside in the lower part of the ice chest, at a tem- 

 perature of from 16° to 19° C, for five hours in order to allow the 

 blood to coagulate. 



Thirty-five cubic centimeters of the clear serum were then drawn 

 off, and to it was added an equal volume of physiological salt solu- 

 tion. 



The mixture was transferred with all due precautions to the inside 

 of a Pasteur-Chamberland B bougie and filtered from within out- 

 ward by means of vacuum, which was applied to the outer surface 

 of the filter in a reverse manner to that shown in fig. 2, omitting 

 the paraffin cup. 



It required one hour to obtain 13.5 cc. of filtrate, which was used 

 for the injection of three nonimmunes, Bonifacio Orea, German 

 Ramos, and Guadalupe Gomez. 



Orea and Ramos each received 5 cc. of the filtrate, injected intra- 

 venously into one of the veins of the arm. As the same was diluted 

 with an equal volume of salt solution, each man received 2.5 cc. of 

 the original blood serum. 



Gomez received 3.5 cc. of the mixture, representing 1.75 cc. of the 

 original serum. 



These injections were made at 4 p. m. September 15, which was 

 just eight hours. after the blood had been drawn from Cruz, the 

 blood having kept five, hours at a temperature of 16° to 19° C. and 

 three hours at room temperature. 



