73 



of 16 generations in fowls ; it resisted 60° C. for three hours, and 

 after one hour in a vacuum tube at 100° C. it was virulent. 



Hydrophobia.— Eemlinger and Eiffat-Bey a ground up a rabbit's 

 brain in water together with a bouillon culture of chicken cholera 

 and filtered it by aspiration tjirough a Berkefeld V filter. The fil- 

 trate inoculated into rabbits caused rabies. 



Celli and de Blasi * ground the brain and spinal cord in sand under 

 300 atmospheres pressure. A suspension in distilled water, when sub- 

 jected to a small Berkefeld filter under a vaccuum of 570 mm. for 

 half an hour, gave an infective filtrate. 



Eemlinger (Ann. de l'lnstitut Pasteur, v. 17, No. 12, 1903, p. 834) 

 confirmed his earlier work with Eiffat-Bey mentioned above. He 

 showed that the virus of hydrophobia can not be made" to pass 

 through a Chamberlajid filter nor through a Berkefeld N or W. 

 It can only be forced through a Berkefeld V, which filter is the most 

 porous of the Berkefeld system. 



Hog cholera. — De Schweinitz," in a preliminary note, mentions a 

 disease peculiar to hogs, indistinguishable clinically and at post- 

 mortem from hog cholera, but which can be transferred from hog to 

 hog by inoculation with certain body fluids which have been rendered 

 free from bacteria by filtration through the finest porcelain filters. 

 This filtrate was shown to contain no organisms of hog cholera or 

 swine plague, because when inoculated into rabbits and guinea pigs 

 the animals remained healthy. 



Rinderpest. — Nicolle and Adil-Bey d passed the virus of this dis- 

 ease through a Berkefeld filter, but not through a Chamberland F. 



Glavelee (sheep pox). — Borrel e filtered a suspension of the pus- 

 tules in water. The filtrate from the Berkefeld filter was infective, 

 but that from the Chamberland F was not. 



Nonfilter ability of vaccine and smallpox. — Parke f crushed vaccine 

 virus with fine sand, using 25 tons of pressure to the square inch. 

 One portion of the suspension of crushed virus was passed through a 

 Berkefeld filter and another portion through a Chamberland filter. 

 Both filtrates were evaporated over sulphuric acid in a vacuum. 



Calves and rabbits inoculated with the filtrate before and after 



" Remlinger and Riffat-Bey : Le virus rabique traverse la bougie Berkefeld. 

 Compt. rend. heb. des Sec. de la Soc. de Biol., vol. 55, 1903, p. 730. 



& Deut. med. wochenschr., vol. 29, p. 945. 



o U. S. Dept. of Agriculture, Bur. Animal Industry, Circular No, 41, Sept., 1903. 



«* Nicolle et Adil-Bey : Etudes sur ia peste bovine. Ann. de l'lnst. Pasteur, 

 vol. 16, 1902, p. 56. 



e Borrel : Experience sur la filtration du virus claveleux. Compt. rend., Soc. 

 de Biol., vol. 54, 1902. 



f Assn. Am. Physicians : Trans., vol. 17, 1902. 



