77 



capable of reproducing the symptoms of the disease when injected 

 into the veins of other men. 



We are not unmindful of the fact that chemical substances derived 

 from the hemoglobulin or other proteids in the blood may be toxic, 

 and we are of course familiar with the work of Gauldi, Montesano, 

 Mannaberg, Celli, and others, Who failed to demonstrate a pyrogenic 

 toxin in malarial blood from similar experiments. The length of 

 time the blood was exposed to the air between the time it was drawn 

 from the malarial patient until it was injected into the person experi- 

 mented upon may account for the discrepancies in results. The time 

 the blood is drawn in relation to the paroxysm and many other factors 

 should also be taken into account. 



Mannaberg drew blood during the attack in a case of ordinary 

 tertian malaria. He centrifugalized it and injected the clear serum 

 subcutaneously into two healthy people. 



One received 1 cc. of serum at 4 p. m., when his temperature was 

 36.7° C. The temperature at 4.30 p. m. was 37° and at 6 o'clock 36°. 



The other patient was given 0.7 cc. of the serum and his temper- 

 ature rose within fifteen minutes after the injection from 36.5° to 

 37.6° C. 



Celli B took during the cold stage a small quantity of blood from 

 each of many malarial patients. 



Young children were inoculated with 50 cc. of the serum sub- 

 cutaneously and 50 cc. intravenously. 



Another child was given the concentrated serum remaining after 

 treating 260 cc. of serum in a vacuum apparatus at low temperature. 

 The child was injected intravenously and subcutaneously. 



From a hemorrhage in a case of severe comatose pernicious malaria 

 25 cc. of serum were obtained and injected into another patient. 



None of the patients into whom the serum was injected showed 

 pyrexia. There was in several instances, however, a slight rise of 

 temperature which the experimenter says may occur after the injec- 

 tion of normal serum. 



Eievel and Behrens c studied a sarcosporidium of the llama. They 

 removed ten of the sacks and ground them up with physiological salt 

 solution in a mortar, and injected 2 cc. of the fluid subcutaneously 

 into a rabbit. After seven hours the rabbit died. The autopsy 

 revealed nothing unusual. 



a Mannaberg, Julius : Die malaria krankheiten. Nothnagel's Specieile Path- 

 ologie und Therapie, Bd. 2, 1899. 



» Celli, Angelo: Malaria. Transl. by J. J. Eyre. Longmans, Green & Co., 

 New York and London, 1900. 



o Rievel and Behrens : Beitrage zur Kenntnis der Sarcosporidien und deren 

 Enzyme. Centralblatt fur bakt. u. parasit. (orig.). Bd. 35, no. 3, s. 341. 



