78 



Another rabbit received by mouth the contents of several sacks 

 rubbed up in salt solution. This rabbit was given at the same time 

 a subcutaneous injection of 1 cc. of the fluid. The animal died after 

 eight hours. From a gross examination of his internal organs and 

 a bacteriologic examination of the same, nothing abnormal was found. 



Blood from the spleens of the above-mentioned rabbits, when 

 injected into three other rabbits, caused no abnormal symptoms. 



Pieces of the flesh of the llama were cut up in salt solution and 

 the fluid part was injected into two rabbits subcutaneously. Both 

 remained sound. 



A rabbit inoculated subcutaneously with a suspension of the con- 

 tents of sarcosporidia sacks in salt solution died after seven hours. 

 The post-mortem was negative. 



Two other rabbits treated in the same way remained alive six 

 hours. Another died after seven hours. 



A suspension was subjected to dialysis and it was found that the 

 dialysat, when injected into a rabbit, caused death within twenty- 

 four hours. The cooked dialysat was inactive. 



Our experimental cases in malaria follow : 



Filtration Experiments with Estivo-Atjtumnal Fever. 



Filomena Martinez (case LXIII), 35 years old, born in Mexico 

 City, lived in Vera Cruz about one year. 



The patient was admitted to the hospital of Working Party No. 2 

 October 27, at 10 a. m. He had been under observation the previous 

 day and early that same morning at San Sebastian Hospital. 



As he showed a heavy infection with malarial parasites he was 

 transferred to our laboratory. 



He gives a history of having had yellow fever about six months ago. 

 His present illness, according to his statement, began some two weeks 

 ago with fever, but he says he did not have chills. The patient's 

 mental condition when seen was below par, and he was unable to give 

 consistent answers. He seemed somnolent and was evidently begin- 

 ning to show the effects of his infection upon the brain. 



An examination of his blood, taken at 4.15 p. m., October 26, 

 showed very many young ring forms, some of them with active 

 amoeboid shapes. None appeared pigmented in the smears stained 

 with Goldhorn's polychrome methylene blue. Crescents and ovoids 

 also present. 



At 12 o'clock noon, October 27, a trifling incision was made through 

 the skin over the median cephalic vein on the right side. A needle 

 was introduced into the vein and 100 cc. of blood were quickly drawn 

 into a sterile flask. The wound was covered with a sterile dressing 

 and healed without complications. 



