79 



The blood was immediately put into the ice chest, the temperature 

 of which registered between 16° and 19° C. Clotting took place 

 rapidly. The red cells settled to the bottom of the flask, the upper 

 part of the clot being composed of a firm yellowish buffy coat. The 

 serum separated well and was very clear. 



The blood serum was drawn off and diluted with an equal volume 

 of an isotonic salt solution and then divided into two portions. 



Temperature chart of Filomena Martinez. 



One portion was passed . through a Chamberland B filter and in- 

 jected into Jose Ojeira. 



The other portion was passed through a Berkefeld filter and 

 injected into Luis Peredo. 



Blood smears made from the blood which was drawn from the vein 

 showed in stained specimens crescents and the young small ring forms 

 of estivo-autumnal malaria. Some of the stained parasites showed 

 one chromatin point, others two. A few were irregular in outline, 



