84 



This filtration was also done from without inwards, as may be seen 

 by reference, to the sketch (fig. 3), but by using vacuum instead of 

 direct air pressure, as in the case of the filtration through the Pasteur- 

 Chamberland bougie. The filtrate was carried over to a sterile bottle, 

 as shown in the sketch, and drawn out with a pipette, so that, care 

 being taken, there could be no chance of contamination. It required 

 about twenty minutes to filter 46.5 cc. of diluted serum, and at 

 8.15 p. m., viz, eight and one-fourth hours after the blood was drawn, 

 twenty cubic centimeters of this filtrate, which represented 10 cc. of 

 the original serum, were injected into the left median basilic vein of 

 Luis Peredo with entirely negative results. 



This filter was then thoroughly washed with water which ran 

 through in drops under atmospheric pressure, and was preserved 

 for further testing, with the following results: 



On March 1, 1904, this filter was tested with a bouillon culture of 

 coccus pyogenes aureus. The filtrate remained sterile after ten days in the 

 incubator. 



At 10 p. m. Martinez was given 1 gram of bimuriate of quinine 

 directly into one of the veins of his arm. This was repeated at noon 

 on October 28, and again at 11 p. m. on the same day. 



On the 29th he received another gram into the vein, with marked 

 improvement in his symptoms and a notable reduction in the number 

 of intracorpuscular forms in the blood, as will be seen by the following 

 notes of the case : 



October 27, 10 p. to. — One gram bimuriate of quinine intravenously. 

 Blood examination, 3 crescents in one field ; some fields have as many 

 as half a dozen small ring forms. 



October 28, 2 a. m. — Blood examination. Some fields have 5 and 6 

 small ring forms, some irregular amoeboid shapes, also ovoids. 



6 a. to. — Blood examination. One or two ring forms to each field; 

 also, crescents. 



10 a. m. — Blood examination. About 1 organism to each field; 

 some young ring forms ; some amoeboid shapes ; also ovoids. 



12 noon. — One gram bimuriate of quinine intravenously. 

 2 p. m.— Blood examination. About 1 intracorpuscular form to 

 each field. 



11 p. to.— One gram bimuriate of quinine intravenously. 

 October 29, 2 a. to.— Ten-minute search of a slide stained with 



polychrome methylene blue showed no intracorpuscular forms ; cres- 

 cents not diminished in numbers. 



6 a. to.— Only 2 intracorpuscular rings seen ; crescents in moderate 

 numbers. 



10 a. to.— Given 1 gram bimuriate of quinine into a vein. Five- 

 minute search of a stained blood smear shows 5 ovoids and only 1 

 intracorpuscular ring form. 



