86 



, Present illness dates from about November 3, but states that he 

 has been troubled with mild attacks of fever for a month, which he 

 describes as coming on alternate days, but not sufficiently severe to 

 keep him from his work. 



The fever which initiated his present sickness began with a severe 

 chill, and was followed by fever and sweat, and was associated with 

 some nausea and vomiting. He states that these paroxysms were 

 repeated daily until his admission to San Sebastian Hospital, Novem- 

 ber 6, 1903. 



Blood examination showed that he had a heavy infectiqn with 

 tertian parasites, and he was immediately transferred to the labora- 

 tory of Working Party No. 2, Yellow Fever Institute. 



The man was physically robust, but very anemic, mucous mem- 

 branes particularly pale, skin cold and damp. 



At about noon on this date (November 6), the patient was seized 

 with a chill. 



By 12.30, half an hour later, the rigor was very marked ; he lay in 

 bed with a blanket drawn over his head, and was shaking violently; 

 he could not hold a thermometer in his mouth, and the pulse was 

 taken with difficulty. During this time the temperature was rapidly 

 rising, it being now 39.1° C. 



At 12.40 blood was drawn from one of the superficial veins at the 

 bend of the elbow. On account of the rigor there was some difficulty 

 in introducing the needle. The blood flowed freely; 125 cc. were 

 quickly drawn. It was permitted to flow into a porcelain dish and 

 immediately defibrinated by whipping with sterilized forks. Clotting 

 took place very quickly, so that the fibrin was readily and quickly 

 separated from the fluid. 



Judging from the size of the clot and color the fibrin had enmeshed 

 a number of corpuscles. The defibrinated fluid showed no further 

 tendency to clot, and on microscopical examination looked like fresh 

 blood containing a normal number of corpuscles. 



To 25 cc. of defibrinated blood was added 25 cc. of physiological 

 salt solution, and this diluted blood was filtered through the same 

 Berkefeld filter in the same manner as was done with the blood of 

 Filomena Martinez (see p. 84). This filter, when tested later, March 

 1, 1904, held back Staphylococcus pyogenes aureus. 



Nine cc. of the filtrate were injected into the right basilic vein of 

 Luis Peredo as soon as this amount could be obtained. This injection 

 took place at 1.40 p. m. It only took about forty minutes to defibrin- 

 ate and filter the blood, which process was done as rapidly as possible. 



Stained smears of the filtrate showed no morphologic elements. 

 The filtrate had a distinct red color. For the method by which this 

 filtration was done, see fig. 3. 



As a control, Jose Ojeira, at 2 p. m., was given an injection into 



