88 



his left basilic vein of 4 cc. of the unflltered mixture. As the blood 

 was diluted with equal parts of salt solution, he therefore received 2 

 cc. of Mendez's blood. 



The unfiltered mixture of defibrinated blood and salt solution, upon 

 microscopic examination shortly after Ojeira received his injection, 

 showed amoeboid tertain organisms with dancing pigment. For the 

 character of the malarial parasites infecting the blood of Mendez, see 

 illustration, plate 3. 



After drawing the blood from Mendez he continued to have a 

 chill, with severe rigor and chattering of the teeth, accompanied by 

 nausea and vomiting. His temperature continued to rise after the 

 blood was drawn until it reached 40.2° C. The febrile period was 

 followed by drowsiness and moisture of the skin. 



As will be seen by reference to the temperature chart, Mendez was 

 kept under observation without quinine, and had another typical 

 malarial paroxysm the next day. All the evidence in his peripheral 

 blood, which was examined frequently, pointed to a, severe double 

 infection with the tertian parasite. 



He was then given quinine, which entirely controlled the disease, 

 and caused the complete disappearance of the parasites from his 

 peripheral blood. 



The results caused by the injection of the blood of Andres Mendez 

 into Peredo and Ojeira follow : 



Luis Peredo (case LXIV), a volunteer, aged 25, born in Jalapa, 

 State of Vera Cruz, where he has always lived. When examined at 

 Jalapa, August 26, he was found to be physically sound; urine con- 

 tained, no albumin; peripheral blood showed no plasmodium. 



He was brought to Vera Cruz August 28 and taken from the station 

 directly to the laboratory, from which time he was kept constantly 

 within a mosquito-proof room. 



On October 27, after having been under daily observation two 

 months, during which time he remained in normal health, he was 

 injected with the filtered blood of Filomena Martinez (page 84), who 

 at the time was suffering with a paroxysm of malarial fever of the 

 estivo-autumnal type, his blood containing many young ring-forms 

 and crescents. 



It will be noted by reference to the records of Filomena Martinez 

 that the blood was drawn during the decline of the paroxysm. It was 

 then allowed to clot in the ice chest ; the clear serum was pipetted off 

 and diluted with an equal quantity of isotonic salt solution, and this 

 filtered through a new Berkefeld filter. 



Twenty cubic centimeters of the filtrate, which on account of the 

 dilution represented 10 cc. of the blood serum, were injected into the 

 left median basilic vein of Peredo. 



