91 



On August 28, at 9.30 a. m., he was bitten by four mosquitoes, two of 

 which had bitten Antonio Leal (case XXXV), a yellow-fever patient 

 fifteen days seventeen hours previously ; and the other two had bitten 

 the same case fourteen days twenty-three hours previously. 



The man was kept under close observation in a mosquito-proof 

 room, but showed no reaction. There was no rise of temperature, nor 

 did he present any untoward symptoms. 



On October 27, 7.20 p. m., he received intravenously 20 cc. of diluted 

 blood serum of Filomena Martinez (sestivo-autumnal infection), 

 passed through a Pasteur- Chamber land filter B. This represented 

 10 cc. of blood serum. For detals of this filtration see Filomena 

 Martinez (p. 81). 



Ojeira showed no reaction whatever as a result of this injection. 



It will be noted that the blood of Martinez was drawn after the 

 height of the paroxysm, and while the temperature was on the decline. 

 Martinez was suffering with a very severe sestivo-autumnal infection 

 at the time the blood was taken. 



Ojeira 's blood was carefully examined several times daily, both be- 

 fore and following this experiment, and at no time was anything 

 resembling a malarial parasite seen in his peripheral blood. 



On November 6, the patient having continued in good health since 

 the last experiment, was used as a control for the experiment made on 

 Peredo. 



On this date, at 2 p. m., he was given an intravenous injection of 

 4 cc. of the unfiltered, diluted, and defibrinated blood of Andres 

 Mendez. At the time the blood was drawn from Mendez it contained 

 a heavy infection of a double tertian malaria, arid the blood was taken 

 from him during a chill and before the height .of his paroxysm. It 

 was at once defibrinated, diluted with an equal volume of physiolog- 

 ical salt solution, and filtered through a Berkefeld filter. Nine cc. of 

 the filtrate were given intravenously to Peredo, causing a malarial 

 paroxysm without, however, the presence of the malarial parasite, 

 and due, as we believe, to the toxin (?) in the blood of Mendez. 



Ojeira, who received 2 cc. of unfiltered blood (4 cc. dilution) , reacted 

 within an hour, with a slight rise of temperature and nausea, and 

 four days following developed a typical malarial paroxysm, with 

 many tertian parasites in his peripheral blood. 



There can be no doubt that the reaction to the 2 cc. of defibrinated 

 blood injected into the vein of Ojeira caused a slight paroxysm, 

 which it is reasonable to suppose was due to the same poison present in 

 the blood of Mendez, and which also caused the reaction in Peredo. 



It will be noticed that 2 cc. of this blood caused but a slight reac- 

 tion in the case of Ojeira, while 4.5 cc. caused a more marked reac- 

 tion, with a rise of temperature to 38.7° C, in the case of Peredo, 



