92 



indicating in a very definite manner that the severity of the symp- 

 toms were directly due to the quantity of poison introduced. Ojeira 

 did not, have a chill or other manifestations of a malarial paroxysm, 

 other than a rise of temperature and nausea. He vomited gastric 

 mucus several times. 



On November 10, the fourth day following the injection, Ojeira 

 had a typical malarial paroxysm, with tertian parasites in his periph- 

 eral blood. He suffered with a double infection, having a chill 

 every day, as will be noticed by reference to the temperature chart. 



The character of the parasites in his blood and the clinical course 

 of the disease resembled in all respects those of Mendez, from whom 

 the blood was taken. Both cases were entirely controlled by quinine. 



MISCELLANEOUS OBSERVATIONS ON MOSQUITOES. 



In association with Doctor Goldberger we made some miscellaneous 

 observations upon the life history and biology of the Stegomyia 

 faseiata and Culex pipiens, some of which were of sufficient interest 

 to record. 



We found that the female Stegomyia does not always lay her eggs 

 at one time. More often she deposits them in groups at intervals of 

 several days. The maximum number laid by any one insect observed 

 by us was 101. The female Stegomyia sometimes dies, apparently 

 from exhaustion, after laying her eggs, but we noted several instances 

 in which this was not the case. The insect which laid 101 eggs was 

 alive and vigorous until killed by us five days later. 



The manner in which the female Stegomyia lays her eggs is of 

 some interest. She bends her abdomen ventrally, and as the genital 

 orifice comes in contact with the sides of the vessel or with the water 

 or other object the egg is deposited. The insect moves along and 

 repeats the performance. 



The eggs are sometimes laid on the water, sometimes on the side 

 of the vessel above the water line, and sometimes on a leaf floating on 

 the water. 



In accordance with the few observations which we made on this 

 particular point, Stegomyia faseiata females that are fed solely on 

 banana or sugar do not lay eggs. They seem to require a feeding of 

 blood for ovipositing. Unconjugated females also do not lay eggs. 



The longest life that we observed was in a female Stegomyia, 

 which we kept for sixty-four days, and then killed for sectioning. _ 



We found, as has been often noted by other observers, that the 

 greatest activity of the Stegomyia faseiata is during the daytime, 

 but we have observed them flying at night. We have also observed 

 them feeding on us at night by artificial light. We have noticed on 

 several occasions that they are especially voracious early in the morn- 



