95 



illness. She was subsequently fed on sirup. On November 1, placed in a cage 

 with a beaker of water and two males. She laid 12 eggs on November 4, which 

 subsequently hatched. 



(Mosquito LVIII-32. Sesoleda Martinez, Rx. b.) This female Stegomyia^ 

 was separated from the breeding jar October 19. Fed upon the blood of Sesoleda T' 

 Martinez, a fatal case of yellow fever October 20, the fourth day of his illness.;;, 

 Subsequently this insect was given banana. October 28 a normal blood feed. 

 On November 5 a beaker of water was placed in the cage to tempt ovipositing. 

 Four days later, November 9, s'he laid 2G eggs, which subsequently hatched. 



The statement has been made that the female Stegomyia fasciata, 

 and mosquitoes generally, require a feeding on blood in order to lay 

 eggs. In three experiments tried by us Ave are able to confirm this 

 statement so far as the Stegomyia fasciata is concerned. The insects 

 were fed on sirup and banana, but could not be tempted to lay eggs. 



Observations. — Banana feeding. A large number of male and female Stego- 

 myia fasciata that had been fed on banana for fourteen days were given a 

 beaker of water to tempt ovipositing. They were left nine days. No eggs laid. 

 They were then billed for section. t 



Sirup feeding. A large number of male and female Stegomyice that had been 

 fed on sirup for fourteen days were given a beaker of water to tempt ovipositing. 

 They were observed twenty-one days later. No eggs were laid. 



Banana and sirup feeding. A large number of male and female Stegomyia', 

 were given alternate feedings of banana and sirup for thirty-two days, at which 

 time a beaker of water was placed in their cage to tempt ovipositing. They 

 were observed nine days later. No eggs were laid. 



Unconjugated females do not lay eggs. 



Observations. — Stegomyiw pupa? were isolated and placed in separate small 

 bottles so that the imagoes could not be kept in strict quarantine. Six of these 

 unconjugated females were given a feeding of blood twenty-four hours after 

 birth, and were subsequently fed on banana. They were kept in a cage with a 

 beaker of water to tempt ovipositing. Five days subsequently they were given 

 a second feeding on blood. Twenty-five days later they were killed, not having 

 laid eggs. 



Size or Screening. 



It is of considerable practical importance in quarantine and public 

 health work to know the size of screening that will keep out the 

 Stegomyia fasciata, and as no accurate observations upon this subject 

 had been made, with which we were familiar, we conducted a few 

 experiments to determine this point. 



Screens with a varying number of meshes to the inch were placed 

 over breeding jars, and banana, sirup, and other food placed on the 

 other side so as to tempt the hungry insects to pass through. These 

 experiments were arranged by placing the fruit and food in a jar 

 which was inverted over the breeding jar. A piece of gauze or net- 

 ting was inserted between the two jars so that the Stegomyice would 

 have to pass through its meshes in order to apear in the upper jar. 



We found that both male and female Stegomyice may pass a wire 



