YELLOW FEYEE INSTITUTE. 



Treasury Department, Bureau of Public Health and Marine-Hospital Service. 

 WALTER WYMAN, Surgeon-General. 



Bulletin No. 15. 



Section B.— ETIOLOGY. P. A. Surg. M. J. ROSENAU, Chairman of Section. 



ATTEMPTS TO GROW THE YELLOW FEVER PARASITE. 



THE HEREDITARY TRANSMISSION OF THE YELLOW FEVER PARASITE IN 



THE MOSQUITO. 



By M. J. Rosenau, Passed Assistant Surgeon, 

 and 

 Joseph Goldbeeger, Passed Assistant Surgeon, 



U. S. Public Health and Marine-Hospital Service. 



ATTEMPTS TO GROW THE YELLOW FEVER PARASITE. 



In view of the fact that the infective principle causing yellow fever 

 may pass through the close-grained pores of a Pasteur-Chamberland 

 B filter, it seemed to us hopeless, with the limitations of the present 

 microscope, to expect to see the causative agent of this disease by 

 direct examination of the blood. 



Novy's work with trypanosomes, both his success in their artificial 

 cultivation and his filtration experiments indicating the possibility of 

 an "ultramicroscopic" phase in their developmental cycle, suggested 

 to us the possibility of cultivating by similar methods the yellow fever 

 parasite and thus perhaps developing a stage in its life cycle which 

 might readily be visible. We attempted, therefore, to grow the para- 

 site of yellow fever in the "water of condensation" of blood-agar 

 tubes. 



The culture tubes were prepared in several different ways; both human 

 blood (nonimmune) and rabbit's blood were used. Some tubes were 

 prepared with the whole, and some with the defibrinated blood. When 



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