109 



to sting the same patient, new about 56 hours after the onset. On 

 October 14, at 3.55 p. m., this insect was made to sting a second yel- 

 low fever patient (Kippers), suffering from a severe attack of the 

 disease, about 48 hours after the onset of the first symptoms." In all, 

 then, this mosquito had three feedings of yellow fever blood from two 

 severe cases in the early stages of the disease. This, as well as all 

 our mosquitoes, was kept in a room in which the temperature was 

 artificially kept between 80° F. and 90° F. Four or five times through- 

 out the course of these experiments the temperature in this room fell 

 to 70° for six or eight hours at a time. 



Group I. — On October 17, at 8.30 a. m. (13£ days from the first, 11^ 

 days from the second, and about 2^ days from the third feed of yellow 

 fever blood), this mosquito deposited eggs from which there were 

 hatched between November 4 (4 p. m.) and November 5 (8.30 a. m.) 

 two* adult female Stegomyia fasciata, which comprise our Group I of 

 Set 1, and were numbered 1 and 2. 



Group II. — On October 19, at 4.30 p. m. (16 days from the first, 14 

 days from the second, and about 5 days from the third feed of yelloW 

 fever blood), this same mother insect laid a second batch of eggs from 

 which were hatched between November 7 (8 a. m.) and November 8 

 (8.30 a. m.) two adult female Stegomyia fasciata, which comprise our 

 Group II of Set 1, and were given the numbers 3 and 4. 



Set 2, Group I, mosquito No. 5. 



Mother mosquito. — The mother mosquito of this set was one of a 

 number raised from larvae and for some time fed on immune blood. 



On October 4, at 9 a. in., this insect was made to sting a severe case 

 (Melancon) of yellow fever 30 hours after the initial chill/ 



Group 7. —October 19, at 4.30 p. m. (15 days after the feed of yellow 

 fever blood), this insect laid a batch of eggs from which there was 

 hatched between November 7 (8.30 a. m.) and November 9 (8.30 a. m.) 

 one adult Stegomyia fasciata, which was given the number 5 and was 

 the only one comprised in this set. 



Set 3, Groups I, II, III, and IV, mosquitoes JVos. 7, 8, 9, 10, 11, 12, 



13, and U. 



Mother mosquito. — The mother mosquito of this set was one of a 

 number of Stegomyia fasciata raised from larvae in our laboratory 



Ti — ; ■ ■ : — -_ - 



«On Oct. 8, 10, and 12, it was permitted to fill itself by stinging an immune. 

 After Oct. 16, fed with sirup. 



» We mention only the mosquitoes that survived and were used for inoculation; 

 others, including males, are ignored in this report. 



« On Oct. 6, 8, 10, 12, and 15, fed by stinging an immune. After Oct. 16, fed with 

 sirup. 



