110 



and, without any prior feeding, was made to sting on October 4, at 

 9 a.m., a severe case of yellow fever (Melancon) 30 hours after the 



initial chill." 



Group. I— October 18, at 4.20 p. m. (14 days after the feed of yel- 

 low fever blood), this insect deposited some eggs, from which there 

 was hatched November 12, at 9 a. m., an adult female Stegomyia 

 fasciata, which formed Group I of this set and was given the num- 

 ber, 6. 



Group II— October 19, at 4.30 p. m. (15 days after the feed of yel- 

 low fever blood), this mother mosquito laid another batch of eggs, 

 from which there were hatched between November 7 (8.30 a. m.) and 

 November 9 (8.30 a. m.) three female Stegomyia fasciata, numbered 

 7, 8, and 9, which form Group II of this set. 



Group III.— November 12, at 9 a. m., there was hatched an addi- 

 tional adult female Stegomyia fasciata, which was numbered 10 and 

 forms Group III of this set. • 



Group IV.— Between November 13 (8.30 a. m.) and November 18 

 (8.30 a. m.) there were hatched four additional females, numbered 

 11, 12, 13, and 14, which comprise Group IV of this set. 



At stated intervals these fourteen Stegomyia fasciata, the progeny of 

 mothers (Sets 1, 2, and 3) that had fed on cases of yellow fever as 

 above indicated, were made to sting nonimmune subjects, and during 

 the intervals fed upon sirup. 



Mosquitoes Nos. 1 and 2 (Set 1, Group I) were applied to and stung 

 seven nonimmunes (A, B, C, D, G, H, K) at various invtervals 

 between the sixth and thirty-ninth day after completing their meta- 

 morphoses, as shown in Table 2. 



Table 2. — Inoculation with mosquitoes Nos. 1 and 2. 

 [Nonimmunes are designated by capital letters.] 



Of these inoculations, four were made after the twenty-second day 

 (the mosquito incubation period in the successful case reported by 

 Marchoux and Simond), namely, at age periods of 24, 32, 35, and 39 

 days. 



«On Oct. 6, 8, 10, 12, and 14, fed by stinging immune. After Oct. 15, fed with 

 sirup. 



