Ill 



Mosquitoes Nos. 3 and i (Set 1, Group II) were made to sting eight 

 nonimmune subjects (B, E, F, G, J, L, M, and N) ten times at inter- 

 vals between the thirteenth and forty-ninth day after emergence as 

 adult insects, as shown in Table 3. 



Table 3. — Inoculation with mosquitoes Nos. S and 4- 

 [Nonimmunes are designated by capital letters.] 



Mosquito. 



Nos. 3 and 4. 



No. 3 



No. 4 



No.3 



Nos. 3 and 4 



Stuug 

 nonim- 

 mune — 



B 

 E 

 E 

 F 

 G 



Age of 

 mosquito 

 at time of 

 stinging. 



Days. 

 13-14 

 21-22 

 23-24 

 26-27 

 29-30 



Mosquito. 



No.3 

 No.3 

 No.3 

 No.3 

 No.3 



Stung 

 nonim- 

 mune — 



J 



oL 



M 



Q 



N 



Age of 

 mosquito 

 at time of 

 stinging. 



Days. 

 33-34 

 37-38 

 42-43 

 46-46 

 49-50 



a Subject, passed from observation at the end of 5 days. 



Mosquito No. 5 (Set 2) was made to sting subject F on two separate 

 days; the first time 25 and the second 27 days after reaching the adult 

 stage, 3 and 5 days, respectively, later than in the positive case reported 

 by the French workers as shown in Table 4. 



Table 4. — Inoculations with mosquito No. 5. 

 [Nonimmune is designated by a capital letter.] 



Mosquito No. 6 (Set 3, Group I) was made to sting at age periods of 

 2, 4, 6, 9, and 12 days longer than in the successful case reported by 

 Marchoux and Simond. 



In all, five subjects were used. 



Table 5. — Inoculation with mosquito No. 6. 

 [Nonimmunes are designated by capital letters.] 



Mosquitoes Nos. 7, 8, and 9 (Set 3, Group II) were made to sting 

 subjects B and F. Subject B was stung by all three insects 3 (to 5) 

 days after completing their metamorphoses. Subject F was stung by 



