INDUCTION, DEVELOPMENT, AND HERITABILITY OF FASCIATIONS. 5 
There were also in the field three or four examples of the type in which 
the alteration of form dated from the rosette stage. These individuals 
were always stalwart plants and produced high, strong stems, of which the 
main axis was fasciated from the base. 
In the O. cruciata the fasciations were remarkably well formed. Some 
of them dated in their development from the rosette period, though usually 
the branches did not begin to flatten until 20 cm. above the base. Many 
of them suddenly flattened from the time of the appearance on the stem of 
a hard protuberance, which was usually cylindrical in shape and sometimes 
2.5 cm. in length, but often reduced to a small lump on the stem (plate 11, 
figs. 1, 3, 4, 5). Ordinarily the stem fasciated from the point where the 
protuberance appeared and one or several of the forks were fasciated. If 
there were no forks the whole stem at once banded. There were also ring- 
fasciations, and the three forms appeared on the same plant. 
The description of a typical individual is as follows: 
C. 6. 2.—The plant developed from a rusette which had been kept in a cold-frame 
from the previous fall (1904), and most of the branches showed by the early disarrange- 
ment of the phyllotaxy and by the shape of the stem that the fasciation had started 
during the rosette period. There were in all 10 branches, of which t was normal; 2 at 
the end of the second summer were small fasciated rosettes; 7 were repeatedly bifurcated, 
3 of them eventually into 9 forks. Of these, 3 showed protuberances at the point of 
the first bifurcation. One stem had a heavy callus at its base, covering an old injury. 
In these 1905 plants there were no rings, but they appeared in 1906 on stock from the 1905 
seed. The largest fasciation on the above plant measured 4.8 cm. by 4mm. There was 
frequently a constriction or channel in the stem below, such as appears in the plant in 
plate 1, fig. 1, but not such as to be histologically akin to the grooves in the wild O. 
biennis. 
In the cruciate forms of 1905, on one plant 15 out of 16 branches were 
fasciated; on another, 10 out of 11. For the microscopic study of this 
material there were to be examined simple flat fasciations, ring-fasciations, 
groove-fasciations, and the fasciations associated with the protuberances. 
In normal structure stems of Onagracez are much alike, and it is unnec- 
essary to call attention to small differences. They possess a continuous 
bundle-ring, which is bicollateral in character. The medullary phloem is 
arranged in groups just within and following the line of the xylem. The 
outer phloem is in very small and inconspicuous groups amid a quantity of 
parenchyma cells. There is a stereome ring, and peripheral to this chloren- 
chyma several rows of collenchyma and the epidermis. The medullary 
rays are but 1 cell in width. The pith consists of large parenchyma cells, 
and there are many bundles of raphides of calcium oxalate there and in 
the cortex. 
There is a kind of latex system which consists of parenchyma cells 
which contain a brown secretion, most dense near the phloem, but con- 
spicuous in pith, cortex, and epidermis. Sections stained in Delafield’s 
hematoxylin resulted in a bright purple in the cells about to crystallize, 
in brown or a greenish shade in the latex cells, and in a reddish purple 
