314 



BACTEEIOLOGY. 



is placed in a position so that only a narrow slit remains 



open. By means of a pi- 

 pette or other arrangement 

 25 c.c. of concentrated 

 potash solution (1:4), are 

 -^ added as rapidly as possi- 

 ble. The top is then closed 

 completely and the rubber 

 band and clamps applied 

 as before. 



Laboratory work. — Liquefy 

 four- glucose agar tubes by heat- 

 ing in the water-bath; then allow 

 to solidify in an upright position. 

 When cool make deep stab cul- 

 tures of the 4 pathogenic anae- 

 robic organisms and then place 

 the tubes at 37°. 



If the agar in the tube is 

 less than one inch deep, it will 



Fig. 52. 



Fig. 53- 



Figs. 51-53. The author's apparatus tor the culture of anaerobic bacteria. Fig. 51. 

 Bottle for tube cultures. Fig. 52. Apparatus for Petri dishes or tubes.— gas or pyrogal- 

 late method. Fig. 53. Apparatus for plates or tubes,— gas, pyrogallate or vacuum method. 



